The widely distributed lakes, as one of the major components of the inland water system, are the primary available freshwater resources on the earth and are sensitive to accelerated climate change and extensive human activities. Lakes play an important role in the terrestrial water cycle and biogeochemical cycle and substantially influence the health of humans living in the surrounding areas. Given the importance of lakes in the ecosystem, long-term monitoring of dynamic changes has important theoretical and practical significance. Here, we extracted water body information and monitored the long-term dynamics of Bosten Lake, which is the largest inland lake in China. We quantified the meteorological factors of the study area from the observation data of meteorological stations between 1988 and 2018. The characteristics of climate change and its correlation with the change of area in the Bosten Lake Basin in the past 30 years were analyzed. The major contributions of this study are as follows: (1) The initial water body was segmented based on the water index model Normalized Difference Water Index (NDWI) and Modified Normalized Difference Water Index (MNDWI) with a pre-assigned threshold value. The results were evaluated with the area extracted through artificial visual interpretation. Then we conducted mathematical morphology operators, opening and closing operations, and median filter to eliminate noise to ensure the accuracy of water body information extraction from the Bosten Lake. A long-term water surface area database of the Bosten Lake was established from high-resolution remote sensing images during 1988–2018. (2) Due to the seasonal difference of snow, ice content, and other objects on images, the areadynamics of Bosten Lake in the recent 30 years were analyzed separately in dry season and rainy season. The water surface area of Bosten Lake showed large inter-annual variations between 1988–2018. (3) Based on the assumption that climatic change has more direct effects on lake than human activities, six meteorological factors were selected to analyze the impacts of climate change on the annual mean lake surface area. The result indicated that in the past 30 years, climate conditions in the Bosten Lake Basin fluctuated greatly. We conducted correlations analysis between the areal dynamics of the Bosten Lake and the meteorological factors. Here, the annual average evaporation had the highest correlation with the areal dynamics of Bosten Lake followed by air temperature, precipitation, sunshine hours, and relative humidity, while the annual average wind speed had the weakest correlation.

Landscape ecological risk assessment (LERA) evaluates different types of potential environmental impacts and their cumulative effects, thereby providing policy insights for sustainable regional land-use and ecosystem management. In a departure from existing literature that heavily relies on low-resolution land-use data for LERA at provincial or municipal scales, this study applies high-resolution land-use data to a relatively small research area (county). In addition, this study modifies the evaluation units of LERA from equal-sized grids to watersheds and refines the ecological vulnerability weight on the basis of finer-resolution data. The main findings are summarized as follows: (1) In 2011–2013, nearly 866 ha of land use in Xiapu County changed; moreover, the construction land, which was mainly concentrated in Songgang Street and Xinan Town, increased the most (340 ha). (2) Landscape ecological risk (LER) was roughly maintained, and areas of high ecological risk were mainly concentrated along the coast. (3) The spatial distribution of LER maintained a relatively aggregated pattern, with no trend toward more aggregated or more dispersed change. This study further discusses the relationship between local LER and land-use change and how to balance global and local LER in planning practices.

Background: Since helminths and malaria parasites are often co-endemic, it is important to clarify the immunoregulatory mechanism that occurs during the process of co-infection. A previous study confirmed that dendritic cells (DCs) are involved in the establishment and regulation of the T-cell-mediated immune response to malaria infection. In the current study, distinct response profiles for splenic DCs and regulatory T cell (Treg) responses were assessed to evaluate the effects of a pre-existing Schistosoma japonicum infection on malaria infection. Methods: Malaria parasitaemia, survival rate, brain histopathology and clinical experimental cerebral malaria (ECM) were assessed in both Plasmodium berghei ANKA-mono-infected and S. japonicum-P. berghei ANKA-co-infected mice. Cell surface/intracellular staining and flow cytometry were used to analyse the level of splenic DC subpopulations, toll-like receptors (TLRs), DC surface molecules, Tregs (CD4⁺CD25⁺Foxp3⁺), IFN-γ/IL-10-secreting Tregs, and IFN-γ⁺/IL-10⁺-Foxp3^-CD4⁺ T cells. IFN-γ, IL-4, IL-5, IL-10 and IL-13 levels were determined in splenocyte supernatants using enzyme-linked immunosorbent assay (ELISA). Results: The co-infected mice had significantly higher malaria parasitaemia, compared with the mono-infected mice, on days 2, 3, 7 and 8 after P. berghei ANKA infection. M ono-infected mice had a slightly lower survival rate, while clinical ECM symptoms, and brain pathology, were significantly more severe during the period of susceptibility to ECM. On days 5 and 8 post P. berghei ANKA infection, co-infected mice had significantly lower levels of CD11c⁺CD11b⁺, CD11c⁺CD45R/B220⁺, CD11c⁺TLR4⁺, CD11c⁺TLR9⁺, CD11c⁺MHCII⁺, CD11c⁺CD86⁺, IFN-γ-secreting Tregs, and IFN-γ⁺Foxp3^-CD4⁺ T cells in single-cell suspensions of splenocytes when compared with P. berghei ANKA-mono-infected mice. Co-infected mice also had significantly lower levels of IFN-γ and higher levels of IL-4, IL-5, and IL-13 in splenocyte supernatants compared to mono-infected mice. There were no differences in the levels of IL-10-secreting Tregs or IL-10⁺Foxp3^-CD4⁺ T cells between co-infected and mono-infected mice. Conclusions: A Tregs-associated Th2 response plays an important role in protecting against ECM pathology. Pre-existing S. japonicum infection suppressed TLR ligand-induced DC maturation and had an anti-inflammatory effect during malaria infection not only by virtue of its ability to induce Th2 responses, but also by directly suppressing the ability of DC to produce pro-inflammatory mediators.

Artemisinin resistance in Plasmodium falciparum has emerged as a major threat for malaria control and elimination worldwide. Mutations in the Kelch propeller domain of PfK13 are the only known molecular markers for artemisinin resistance in this parasite. Over 100 non-synonymous mutations have been identified in PfK13 from various malaria endemic regions. This study aimed to investigate the genetic diversity of PvK12, the Plasmodium vivax ortholog of PfK13, in parasite populations from Southeast Asia, where artemisinin resistance in P. falciparum has emerged. The PvK12 sequences in 120 P. vivax isolates collected from Thailand (22), Myanmar (32) and China (66) between 2004 and 2008 were obtained and 353 PvK12 sequences from worldwide populations were retrieved for further analysis. These PvK12 sequences revealed a very low level of genetic diversity (π = 0.00003) with only three single nucleotide polymorphisms (SNPs). Of these three SNPs, only G581R is nonsynonymous. The synonymous mutation S88S is present in 3% (1/32) of the Myanmar samples, while G704G and G581R are present in 1.5% (1/66) and 3% (2/66) of the samples from China, respectively. None of the mutations observed in the P. vivax samples were associated with artemisinin resistance in P. falciparum. Furthermore, analysis of 473 PvK12 sequences from twelve worldwide P. vivax populations confirmed the very limited polymorphism in this gene and detected only five distinct haplotypes. The PvK12 sequences from global P. vivax populations displayed very limited genetic diversity indicating low levels of baseline polymorphisms of PvK12 in these areas.

Background: The malaria elimination plan of the Greater Mekong Subregion (GMS) is jeopardized by the increasing number of Plasmodium vivax infections and emergence of parasite strains with reduced susceptibility to the frontline drug treatment chloroquine/primaquine. This study aimed to determine the evolution of the P. vivax multidrug resistance 1 (Pvmdr1) gene in P. vivax parasites isolated from the China–Myanmar border area during the major phase of elimination. Methods: Clinical isolates were collected from 275 P. vivax patients in 2008, 2012–2013 and 2015 in the China–Myanmar border area and from 55 patients in central China. Comparison was made with parasites from three border regions of Thailand. Results: Overall, genetic diversity of the Pvmdr1 was relatively high in all border regions, and over the seven years in the China–Myanmar border, though slight temporal fluctuation was observed. Single nucleotide polymorphisms previously implicated in reduced chloroquine sensitivity were detected. In particular, M908L approached fixation in the China–Myanmar border area. The Y976F mutation sharply decreased from 18.5% in 2008 to 1.5% in 2012–2013 and disappeared in 2015, whereas F1076L steadily increased from 33.3% in 2008 to 77.8% in 2015. While neutrality tests suggested the action of purifying selection on the pvmdr1 gene, several likelihood-based algorithms detected positive as well as purifying selections operating on specific amino acids including M908L, T958M and F1076L. Fixation and selection of the nonsynonymous mutations are differently distributed across the three border regions and central China. Comparison with the global P. vivax populations clearly indicated clustering of haplotypes according to geographic locations. It is noteworthy that the temperate-zone parasites from central China were completely separated from the parasites from other parts of the GMS. Conclusions: This study showed that P. vivax populations in the China–Myanmar border has experienced major changes in the Pvmdr1 residues proposed to be associated with chloroquine resistance, suggesting that drug selection may play an important role in the evolution of this gene in the parasite populations.

Information about tree species plays a pivotal role in sustainable forest management. Light detection and ranging (LiDAR) technology has demonstrated its potential to obtain species information using the structural features of trees. Several studies have explored the structural properties of boreal or temperate trees from terrestrial laser scanning (TLS) data and applied them to species classification, but the study of structural properties of tropical trees for species classification is rare. Compared to conventional static TLS, handheld laser scanning (HLS) is able to effectively capture point clouds of an individual tree with flexible movability. Therefore, in this study, we characterized the structural features of tropical species from HLS data as 23 LiDAR structural parameters, involving 6 branch, 11 crown and 6 entire tree parameters, and used these parameters to classify the species via 5 machine-learning (ML) models, respectively. The performance of each parameter was further evaluated and compared. Classification results showed that the employed parameters can achieve a classification accuracy of 84.09% using the support vector machine with a polynomial kernel. The evaluation of parameters indicated that it is insufficient to classify four species with only one and two parameters, but ten parameters were recommended in order to achieve satisfactory accuracy. The combination of different types of parameters, such as branch and crown parameters, can significantly improve classification accuracy. Finally, five sets of optimal parameters were suggested according to their importance and performance. This study also showed that the time- and cost-efficient HLS instrument could be a promising tool for tree-structure-related studies, such as structural parameter estimation, species classification, forest inventory, as well as sustainable tree management.