The biosynthesis of algal-based zinc oxide (ZnO) nanoparticles has shown several advantages over traditional physico-chemical methods, such as lower cost, less toxicity, and greater sustainability. In the current study, bioactive molecules present in Spirogyra hyalina extract were exploited for the biofabrication and capping of ZnO NPs, using zinc acetate dihydrate and zinc nitrate hexahydrate as precursors. The newly biosynthesized ZnO NPs were characterized for structural and optical changes through UV-Vis spectroscopy, Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), scanning electron microscopy (SEM), and energy dispersive X-ray spectroscopy (EDX). A color change in the reaction mixture from light yellow to white indicated the successful biofabrication of ZnO NPs. The UV-Vis absorption spectrum peaks at 358 nm (from zinc acetate) and 363 nm (from zinc nitrate) of ZnO NPs confirmed that optical changes were caused by a blue shift near the band edges. The extremely crystalline and hexagonal Wurtzite structure of ZnO NPs was confirmed by XRD. The involvement of bioactive metabolites from algae in the bioreduction and capping of NPs was demonstrated by FTIR investigation. The SEM results revealed spherical-shaped ZnO NPs. In addition to this, the antibacterial and antioxidant activity of the ZnO NPs was investigated. ZnO NPs showed remarkable antibacterial efficacy against both Gram-positive and Gram-negative bacteria. The DPPH test revealed the strong antioxidant activity of ZnO NPs.

Iron oxide nanoparticles (NPs) have attracted substantial interest due to their superparamagnetic features, biocompatibility, and nontoxicity. The latest progress in the biological production of Fe₃O₄ NPs by green methods has improved their quality and biological applications significantly. In this study, the fabrication of iron oxide NPs from Spirogyra hyalina and Ajuga bracteosa was conducted via an easy, environmentally friendly, and cost-effective process. The fabricated Fe₃O₄ NPs were characterized using various analytical methods to study their unique properties. UV-Vis absorption peaks were observed in algal and plant-based Fe₃O₄ NPs at 289 nm and 306 nm, respectively. Fourier transform infrared (FTIR) spectroscopy analyzed diverse bioactive phytochemicals present in algal and plant extracts that functioned as stabilizing and capping agents in the fabrication of algal and plant-based Fe₃O₄ NPs. X-ray diffraction of NPs revealed the crystalline nature of both biofabricated Fe₃O₄ NPs and their small size. Scanning electron microscopy (SEM) revealed that algae and plant-based Fe₃O₄ NPs are spherical and rod-shaped, averaging 52 nm and 75 nm in size. Energy dispersive X-ray spectroscopy showed that the green-synthesized Fe₃O₄ NPs require a high mass percentage of iron and oxygen to ensure their synthesis. The fabricated plant-based Fe₃O₄ NPs exhibited stronger antioxidant properties than algal-based Fe₃O₄ NPs. The algal-based NPs showed efficient antibacterial potential against E. coli, while the plant-based Fe₃O₄ NPs displayed a higher zone of inhibition against S. aureus. Moreover, plant-based Fe₃O₄ NPs exhibited superior scavenging and antibacterial potential compared to the algal-based Fe₃O₄ NPs. This might be due to the greater number of phytochemicals in plants that surround the NPs during their green fabrication. Hence, the capping of bioactive agents over iron oxide NPs improves antibacterial applications.

The application of green synthesis for silver nanoparticles in nanomedicine has experienced significant growth. Strobilanthes glutinosus, a plant primarily located in the Himalayas, remains largely unexplored. Considering the biomedical value of S. glutinosus, phytochemicals from this plant were used for the biosynthesis of silver nanoparticles. Silver nanoparticles were synthesized from aqueous extract of root and leaves of Strobilanthes glutinosus. The synthesized silver nanoparticles were characterized using UV–Vis spectrophotometry, Fourier-transform infrared spectroscopy, transmission electron microscopy, and X-ray diffraction. Total phenolic and flavonoid contents of plants were determined and compared with nanoparticles. The biomedical efficacy of plant extracts and silver nanoparticles was assessed using antioxidant and antibacterial assays. The UV–Vis spectra of leaf- and root-extract-mediated AgNPs showed characteristic peaks at 428 nm and 429 nm, respectively. TEM images revealed the polycrystalline and spherical shapes of leaf- and root-extract-mediated AgNPs with size ranges of 15–60 nm and 20–52 nm, respectively. FTIR findings shown the involvement of phytochemicals of root and leaf extracts in the reduction of silver ions into silver nanoparticles. The crystalline face-centered cubic structure of nanoparticles is depicted by the XRD spectra of leaf and root AgNPs. The plant has an ample amount of total phenolic content (TPC) and total flavonoid content (TFC), which enhance the scavenging activity of plant samples and their respective AgNPs. Leaf and root AgNPs have also shown good antibacterial activity, which may enhance the medicinal value of AgNPs.

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Bacillus megaterium is a gram positive rod shaped bacterium that is greatly responsible for the formation of spores aerobically and found throughout the world. The size of respective bacterium genome is 5.48 Mb and contains about 5,858 exons. It has two strains named as QMB1551 and DSM319 having GC amount 38%. Regardless, a large number of the genes do not have any anticipated function, being depicted as hypothetical proteins. It is one of the most significant industrial bacterium that is employed for the generation of various useful products. It has played a promising role in the field of biotechnology as a production of genetically engineered proteins. Bioinformatics is one of the most advanced field that employed various productive tools in order to analyze the sequence, structure and function of proteins. In order to predict the function of a hypothetical protein of B. megaterium DSM319, BLAST was utilized for sequence comparison of hypothetical proteins of the bacterium dataset against the subject sequence of dataset of biotechnological important proteins. The best hit obtained from BLASTp results for sequence similarity was ABC-2 family transporter proteins having known functions. With that about 80% of similarity a daunorubicin ABC transporter permease (Anaerobacillus alkaliacustris) having query cover 100% and e-value 1 e-153 is obtained. The permease enzyme facilitates the ABC transporter to carry out export of daunorubicin that is produced by Bacillus spp. as a secondary metabolite. From the current study, it is predicted that B. megaterium having a protein known as daunorubicin ABC transporter permease. Moreover, this bacterium might be able to produce daunorubicin as a secondary metabolite. Daunorubicin has a great significance in medical sciences especially in anticancer therapy.