Tree tomato (Solanum betaceum Cav.) is an Andean fruit crop that is grown in Ecuador. It is an exceptional source of minerals and vitamins, thus has nutraceutical properties. The objective of this research was to carry out a phytochemical characterization of a breeding population composed of 90 segregants. Pulp (including mesocarp, mucilage, seeds and placenta) was ground and sieved in order to obtain the liquid pulp to be lyophilized for the chemical analyzes. Antioxidants compounds were determined by spectrophotometry and vitamin C by reflectometry. Data were analyzed by principal components, grouping, and variance analyses; in addition, Z Score estimation was carried out to select elite individuals. There was a broad variability in the data obtained for the breeding population, polyphenol content varied from 5.11 to 16.59 mg GAE g⁻¹, flavonoids from 1.24 to 6.70 mg cat g⁻¹, carotenoids from 50.39 to 460.72 µg β-carotene g⁻¹, anthocyanins from 1.06 to 240.49 mg cy-3-glu 100 g⁻¹, antioxidant capacity from 49.51 to 312.30 µm Trolox g⁻¹, and vitamin C from 78.29 to 420.16 mg 100 g⁻¹. It can be concluded that tree tomato is a good source of beneficial biocompounds and has a high antioxidant capacity.

Salt stress is a major constraint to crop productivity, negatively affecting plant physiology and fruit quality. This study hypothesized that seed priming with polyethylene glycol (PEG6000) might enhance antioxidant activity by mitigating oxidative stress in Solanum lycopersicum ‘Micro-Tom’ under salt stress. Seeds primed with –1.2 MPa PEG6000 were grown in Rockwool and treated with 0, 50, 100, 150, and 200 mM NaCl. Primed plants showed a 32% increase in leaf potassium (K⁺) and a 28% decrease in sodium (Na⁺) accumulation compared to non-primed plants under 150 mM NaCl. Glucose, fructose, and sucrose contents increased by 25%, 22%, and 19%, respectively, in primed fruits, while citric acid decreased by 15%. Malondialdehyde (MDA) and electrolyte leakage were reduced by 35% and 29%, respectively, in primed plants under moderate salinity. Antioxidant enzyme activities—SOD, POD, CAT, and APX were enhanced by 30–45% in primed plants under 100 and 150 mM NaCl, compared to non-primed controls. Abscisic acid (ABA) levels increased by 40% in primed roots under salt stress. Activities of polyamine-related enzymes (DAO, PAO, and ADC) also rose significantly. Priming improved protein content by 20% and relative water content by 18%. These results suggest that PEG6000 seed priming enhances salt tolerance by boosting antioxidant defense, regulating osmotic balance, and improving ion homeostasis, offering a viable strategy for sustaining tomato productivity under salinity.

The excess presence of calcium carbonate (CaCO₃) in soil poses challenges for production of horticultural crops, including tomatoes. This condition is prevalent in arid and semi-arid regions of Afghanistan. The objective of this study was to evaluate the effects of elevated concentrations of CaCO₃ on growth, physiology, and quality attributes of tomato. Seedlings were exposed to different concentrations of CaCO₃ (0%, 2.5%, 5%, 10%, and 20% w/w) in soil. The results showed that elevated concentrations of CaCO₃ (10% and 20%) significantly increased soil electrical conductivity (EC) and pH, and subsequently affected growth, physiology, and quality of tomato. CaCO₃ effects resulted in an increase in leaf electrolyte leakage, leaf calcium content, root respiration rate, root ethylene production, fruit firmness, total soluble solids, ascorbic acid, and organic acids, as well as a decrease in plant height, leaf length, leaf magnesium content, leaf SPAD value, number of leaves per plant, root weight and length, and root activity. At higher concentrations, CaCO₃ decreased number of flowers and fruit per plant, as well as fruit weight and diameter, consequently affecting yield production. Although elevated concentrations of CaCO₃ is characteristic of soils in Afghanistan, limited information is available about this topic. These findings enhance our understanding of soil conditions in the country and provide valuable insights for farmers.

Effective water and soil management is crucial for crop productivity, particularly in rice cultivation, where poor soil quality and water scarcity pose challenges. The response of deeper-rooted rice grown in soils amended with different soil amendments (SAs) to Internet of Things (IoT)-managed alternate wetting and drying (AWD) irrigations remains undetermined. This study explores the effects of various SAs on DRO-1 IR64 rice plants under IoT-based soil moisture monitoring of AWD irrigation. A greenhouse experiment executed at the Tokyo University of Agriculture assessed two water management regimes—continuous flooding (CF) and AWD—alongside six types of SAs: vermicompost and peat moss (S + VC + PM), spirulina powder (S + SPP), gypsum (S + GS), rice husk biochar (S + RHB), zeolite (S + ZL), and soil without amendment (S + WA). Soil water content was continuously monitored at 10 cm depth using TEROS 10 probes, with data logged via a ZL6 device and managed through the ZENTRA Cloud application (METER GROUP Company). Under AWD conditions, VC + PM showed the greatest decline in volumetric water content due to enhanced root development and water uptake. In contrast, SPP and ZL maintained consistent water levels. Organic amendments like VC + PM improved soil properties and grain yield, while AWD with ZL and GS optimized water use. Strong associations exist between root traits, biomass, and grain yield. These findings highlight the benefits of integrating SAs for improved productivity in drought-prone rice systems.

Light availability is a key environmental factor regulating nitrogen assimilation, carbon metabolism, and nutritional quality in leafy vegetables grown in controlled environments. However, how practical lighting regimes used in plant factories with artificial lighting (PFALs) influence the coordination between nitrogen assimilation and central carbon metabolism across different lettuce cultivar types remains insufficiently understood. This study investigated how moderate differences in photosynthetic photon flux density (PPFD) influence nitrogen metabolism and metabolic coordination in hydroponically cultivated lettuce. Two cultivars representing contrasting morphological types, iceberg lettuce (‘Celebration’) and leaf lettuce (‘Sunny’), were grown under LED light intensities of 150 and 200 µmol·m⁻²·s⁻¹. Nitrate, nitrite, and ammonium concentrations were measured together with the activities of nitrate reductase (NRA) and nitrite reductase (NiRA), as well as ascorbic acid content. Metabolomic profiling was additionally performed to characterize broader metabolic responses. Higher light intensity enhanced nitrate reduction capacity in both cultivars, but the resulting patterns of nitrogen accumulation were strongly genotype-dependent. The leaf lettuce cultivar ‘Sunny’ exhibited increased NRA and reduced nitrate accumulation under higher light intensity, whereas the iceberg lettuce cultivar ‘Celebration’ accumulated more nitrate under the same conditions. Ammonium responses further suggested differences in downstream nitrogen assimilation processes. Elevated light intensity also increased ascorbic acid levels in both cultivars. Metabolomic analysis revealed contrasting cultivar-specific shifts in central carbon metabolism, particularly involving soluble sugars and tricarboxylic acid cycle intermediates, indicating differential coordination between carbon metabolism and nitrogen utilization. Overall, these findings demonstrate that moderate changes in light intensity within the practical PFAL cultivation range can significantly influence the integration of carbon and nitrogen metabolism in lettuce. Importantly, cultivar-specific physiological traits determine how these metabolic responses translate into nitrate accumulation and nutritional quality in controlled-environment production systems.

Drought reduces soil moisture and impairs root function, posing a significant threat to rice production in arid regions. The influence of soil amendments on early rice root development under semi-dry cultivation remains insufficiently characterized, especially when assessed using non-destructive rhizotron techniques. This study employed a scanner-based rhizotron system to evaluate early root responses of rice seedlings to six amendments under semi-dry irrigation: vermicompost and peat moss, spirulina powder, gypsum, rice husk biochar, zeolite, and an unamended control. The vermicompost plus peat moss (VC+PM) treatment demonstrated the highest water-holding capacity (26%), root projected area (9.60 cm² plant⁻¹), and root surface area (84.79 cm² plant⁻¹). VC+PM also promoted extensive lateral branching (233 secondary and 1709 tertiary roots) and the greatest total lateral root length (363.09 cm plant⁻¹), resulting in superior biomass (shoot: 140.00 mg plant⁻¹; root: 56.70 mg plant⁻¹) and the lowest root-to-shoot ratio (0.90). These improvements are attributed to the enhanced moisture retention of peat moss and the nutrient and phytohormone contributions of vermicompost. In contrast, rice husk biochar exhibited the lowest water-holding capacity (14%), while other amendments produced moderate or limited effects. The results establish a direct relationship between improved soil water retention and early-stage drought-avoidant root development. The combination of VC and PM emerges as a promising approach to enhance root plasticity and seedling establishment in water-saving rice systems. As this study was conducted under controlled rhizotron conditions and limited to the seedling stage (20 days after sowing), future research should prioritize multi-season field trials to assess yield translation and economic feasibility assessments to support farmer adoption.