Animal feeding research has revealed a close relationship between the chemical composition and nutritional value of cow rations, the number of rumen bacterial communities and animal productivity. Our present research aimed to investigate the outcome of inclusion of different levels of protein concentrate in rations of Ayrshire dairy cows in relation to the rumen microbiome, reproductive traits and economic value. Forty-five Ayrshire cows were divided into three groups (15 in each). The first control group 0 AM was fed the basal ration, while the second 1 AM and third 2 AM groups were fed the basic ration with the sunflower cake replaced by different levels of protein concentrate Agro-Matic (1 and 1.5 kg/head/day, respectively). Ruminal fluid samples, reproductive parameters and economic value were studied. During the early lactation period, 120 days in milk (DIM), the number of pathogenic microorganisms decreased in both the 1 AM and 2 AM groups when compared with the control group 0 AM; moreover, a significant decrease in Peptococcus bacteria was recorded in the 1 AM group, while Fusobacterium decreased in the 2 AM group. At the end of lactation, the total number of cellulolytic bacteria increased with the use of protein concentrate in animals of the 1 AM group when compared with the control group. Regarding undesirable bacteria, the 2 AM group recorded the highest value for Lactobacilli and Actinobacteria when compared with the 0 AM group (0.18 and 8.90 vs. 0.04 and 4.24), and the differences were significant (p < 0.05). The insemination index and the duration of the days open period decreased in the 2 AM group, while the differences were p > 0.05. The profitability of milk production increased by 2.76% and 6.28% in both supplemented groups, and the differences compared to the 0 AM group were significant. We conclude that the supplementation of Agro-Matic caused no deviations from the normal standards of cellulolytic, amylolytic, transit and pathogenic bacteria, no impact on reproductive functions and significantly improved the profitability of the milk production process of Ayrshire dairy cows.

To determine the level of application and the effectiveness of the use of plant feed additives from sweet chestnut wood extract (Castanea Sativa Mill) in the diet of Cobb-500 cross broiler chickens, four groups were formed via the balanced groups method. The chickens in the experimental groups were supplemented with sweet chestnut wood extract in the main diet rather than a feed antibiotic at an amount of 500 g per ton of compound starter feed and 250 g per ton of grower and finisher in the second experimental group; 650 g per ton of compound starter feed and 325 g per ton of grower and finisher in the third experimental group; and 800 g per ton of compound starter feed and 400 g per ton of grower and finisher in the fourth experimental group. Supplementation with phytobiotics led to an increase in the digestibility of the dry matter in the second and third groups compared to the first experimental group. Furthermore, broiler chickens supplemented with a medium dose of phytobiotics revealed a significant difference in both crude protein and fiber when compared to the second experimental group (91.95% and 12.11% vs. 88.98% and 10.07%, respectively). The preslaughter weight of the birds in the phytobiotics supplemented groups was higher than in those fed with the lowest dosage of phytobiotic by 5.47%, and the difference was significant. There were no significant differences in terms of the blood biochemical parameters between the groups. In summary, the inclusion of plant feed additives from sweet chestnut wood extract as a substitute for an antibacterial drug in the diet of broiler chickens did not deteriorate the blood biochemical parameters and improved the intensity of the nutrient digestion process. As a result, it enhanced the quality indicators of the broiler carcass during the entire growth period.

The effect of activated charcoal as a feed additive was assessed in the experiment with Romanov sheep and Romanov sheep breed with argali. Charcoal sorbent is the original preparation produced in “Khiminvest” Science and Technology Center. Activated charcoal supplement was added to the basic diet of experimental animals in an amount of 1 g per 10 kg of live weight. The preparation showed positive effect on the palatability of the feed and ruminal digestion processes, as well as contributed to an increase in dry matter concentration of microorganisms and amylase activity. Experimental sheep showed an increase of digestibility of dry matter, dry protein, crude fat, crude fiber, bio-effecting agents, as well as increased nitrogen utilization coefficient. Use of the preparation provided increase in daily weight gains.

The purpose of this study was to determine the level of application and effectiveness of the use of vegetable feed additives from complex phytobiotic feed additives (CPFA) in the diets of broiler chickens, as well as their effects on growth indicators, carcass characters and blood profile. A total of 258 Ross 308 chicks were divided into six dietary regimens, including: a basal diet without additives as a first control group (CON); the second group received a basal diet supplemented with 200 g/t in the starter phase and 100 g/t in the grower and finisher phase; the third group—400 g/t and 200 g/t; the fourth group—600 g/t and 300 g/t; the fifth group—800 g/t and 400 g/t; and the sixth group—1000 g/t and 500 g/t of a complex phytobiotic supplement based on tannins, respectively. The CPFA contains the following: tannins 36.8–55.2%, eugenol 0.4–0.6%, cinnamon aldehyde 0.8–1.2%, zinc–methionine 1.6–2.4%, calcium butyrate 8–12%, silicon dioxide 1.2–1.8% and dextrose up to 100%. The maximum introduction of phytobiotics (1000 g/t) at 7 days of age leads to a decrease in the live weight of broilers which reduced by 8.27% (p < 0.05) compared to the minimum level of phytobiotics (200 g/t). From 15–21 days, the live weight was significant between the supplemented and control groups and represented 396.21, 384.81 and 384.16 vs. 316.91 g for the CPFA 4, CPFA 5, CPFA 1 and control group, respectively. Furthermore, the same trend was recorded in the average daily gain during the periods between 15–21 and 22–28 days of the experiment. Feeding CPFA had a positive effect on the carcass indicators, except for the feeding of CPFA 3 in the amount of 600 g/t in the starter phase and 300 g/t in the grower and finish phases, which recorded the lowest weight in relation to the CPFA 1 and 2 groups and represented 1309.58 vs. 1460.06 and 1456.52 g, respectively, and the difference was significant. The inclusion of CPFA in poultry diets contributed to an increase in lung mass in the experimental groups relative to the control group, except for the CPFA 5 group which represented the lowest weight of lung mass (6.51 g) and the differences were significant between the CPFA 2 and CPFA 3 and the control groups. The highest concentration of leukocytes was observed during the experiment period in the group of poultry receiving phytobiotics (CPFA 3), which significantly exceeded the control group by 2.37 × 109/L. A significant decrease in the level of cholesterol was recorded in the CPFA groups when compared to the control group and represented 2.83 vs. 3.55 mmol/L, respectively. Consequently, the introduction of vegetable feed additives from complex phytobiotic feed additives (CPFA) in the diets of Ross 308 chicks had a positive effect on the growth production, the carcass yield, the mass of the pectoral muscles and the mass of the lungs. Moreover, it did not cause a harmful effect on the biochemical parameters of the blood.

Our research purpose was to study the effect of the inclusion of a combination of phytobiotics in the form of dry Fucus vesiculosus grits (FG) and a mineral adsorbent from the heat-treated mineral shungite (TMS) on milk productivity, nutrient digestibility, and biochemical parameters of the Suksun dairy cows. A total of 80 dry-hardy cows of the Suksun breed were divided into four groups (20 heads each), balanced primarily by breed, age, body weight, body condition score, and indicators of milk yield for the previous lactation. The selected cows were with an average live body weight of 512.0 ± 1.28 kg, BCS 3.0–3.5, and parities of 6250 kg milk. The control group (CON) were fed the basic ration only; the second (TMS), third (FG), and fourth (TMS + FG) groups were fed the basic ration provided by 50 g of the mineral adsorbent from heat-treated shungite, 100 g of Fucus grits (Fucus vesiculosus), 50 g of the mineral adsorbent from heat-treated shungite, and 100 g of dry grits from Fucus vesiculosus, respectively. The total protein content in milk was significantly higher in the group receiving Fucus vesiculosus by 0.05% and the group receiving a combination of mineral adsorbent and Fucus vesiculosus by 0.03%. The percentage of milk fat content recorded the highest significant value in (TMS) group when compared to the control and represented (4.37 vs. 3.95). The group of cows that received (TMS + FG) revealed a significant difference in the digestibility of both ether extract and crude fiber when compared to the control group and represented (54.74 vs. 51.71 and 60.68 vs. 55.15%), respectively. The cows supplemented with a mineral adsorbent or a combination of mineral adsorbent and Fucus vesiculosus revealed a significant difference in the digestibility of ether extract and crude fiber in the group receiving TMS + FG by 3.0% (p < 0.05) and 5.5% (p < 0.05), respectively. The intake of nitrogen with the diet increased in (FG) and (TMS + FG) groups by 11.3 g (p < 0.05) and 13.4 g (p < 0.05) of nitrogen. There was an increase (p < 0.05) in the concentration of rumen ammonia in the control group compared to the other groups. The glucose content of those cows that received FG and TMS + FG combination increased (p < 0.05) by 0.76 mmol/L and 0.90 mmol/l in relation to the control group. The globulin, albumin/globulin ratio, and the level of triglycerides revealed a significant difference between the different experimental groups. In brief, the inclusion of a combination of phytobiotics in the form of dry Fucus vesiculosus grits and a mineral adsorbent from the heat-treated mineral shungite in Suksun dairy cows’ diets improved milk composition, digestibility of nutrients, utilization of nitrogen, and did not cause deleterious effects on blood biochemical indicators.

The article presents experimental data on the use of different levels of protein concentrate supplement in the diets of lactating cows with a high proportion of indigestible protein in the rumen. During the course of the research it was observed that the inclusion of Agro-Matic protein concentrate in the amount of 1.0 and 1.5 kg per head per day in the diet instead of other protein feeds increased the level of indigestible (by-pass) protein by 6.3 % and 9.1 %, respectively. The introduction of the maximum amount of Agro-Matic protein concentrate into the diet has contributed to the change in the amino acid composition of milk. It was established that the yield of milk protein during the period of milking was significantly higher in animals receiving different levels of protein concentrate compared to those of the control group. Feeding lactating cows diets with different levels of protein concentrate did not have a negative impact on biochemical parameters of animal blood. All measured blood of experimental animals were within the physiological norms. The maximum content of essential amino acids in the milk of cows from 3 experimental groups was significantly higher and reached up to 1560.9 mg% versus 1480.17 mg% in the control group.