This study aims to assess the accuracy and reliability of two different types of thermometers: contact thermometers (CT) and non-contact thermometers (NCIRT) at Maharaj Nakorn Chiang Mai Hospital. The comparison method was carried out in a hospital laboratory setting and followed a specific calibration procedure. The mean readings, errors, and measurement uncertainties were reported and categorized according to the manufacturer’s labeling models. The maximum permissible errors were defined by international standards and clinical acceptances for contact and NCIRTs, respectively. The results showed that thermometers of temperature probes (PTs) provided the highest level of accuracy. When compared to a reference standard thermometer (STD), the mean errors were only 0.04 °C at the calibration point of 37.0 °C. For digital CTs, the mean error at the calibration points of 37.0 °C was 0.24 °C. Most CT models exhibited high accuracy and reliability. However, the forehead (IRFTs) and ear (IRETs) readings significantly differed from the STD, with over half of all units not meeting the ISO 80601–2–56/ASTM E1965–98 standard requirements. These results suggest that CTs and PTs are highly accurate and reliable devices for measuring body temperature. However, both IRFTs and IRETs could be useful in certain situations, such as screening many people in a short amount of time, but their accuracy should be interpreted with caution and confirmed using a more precise method if necessary.

The venom of the Asian hornet (Vespa affinis) comprises a complex mixture of biologically active substances, including various enzymes such as phospholipase A and hyaluronidase; amines such as histamine, serotonin, and catecholamines; peptides such as mastoparan and vespakinin; and other components including acetylcholine and antigen 5. This complexity reflects the highly evolved nature of V. affinis as a venomous insect. The composition of animal venoms often exhibits a certain degree of variability, making the study of biogenic amines particularly intriguing. The objective of this research was to confirm and identify the presence of tetradecylamine in the venom of Vespa affinis using the scientific computational analysis software MetFrag. In addition, the study aimed to construct the biosynthetic pathway of this compound and to predict its potential biological roles. The predicted biosynthetic route of tetradecylamine suggested its possible involvement in antibacterial activity. Antibacterial assays were performed against four bacterial strains Escherichia coli, Staphylococcus aureus, Bacillus cereus, and Klebsiella pneumoniae. The results revealed that tetradecylamine exhibited notable inhibitory effects, with minimum inhibitory concentration (MIC) values of 2, 4, 8, and 4 µg/mL, and minimum bactericidal concentration (MBC) values of 2, 4, 8, and 4 µg/mL, respectively. Furthermore, molecular docking studies were conducted using penicillin-binding protein 2x (PBP2x, PDB ID: 5OIZ) as the target protein. Among eight tested ligands, streptomycin exhibited the highest binding affinity with a docking score of 64.76. In contrast, biogenic amines such as 2-phenylethylamine and tetradecylamine showed docking scores of 33.74 and 48.2, respectively. In the MurA protein (PDB ID: 3VCY), the biogenic amine ligand tetradecylamine exhibited a binding affinity comparable to that of certain reference drugs. Specifically, tetradecylamine achieved a GOLD score of 52.58, whereas ampicillin showed a higher score of 61.53. Notably, tetradecylamine demonstrated a higher binding affinity to the target protein compared with certain conventional antibiotics such as doxycycline and gentamycin.