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Dr. Pedro Pereira
ITQB NOVA
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(Calculated once Scilit publication data is synced: current year − year of first publication + 1)
Representative Publications
View All Publications12 August 2016 in Nature Communications
Journal Article
NGNils Gustafsson
Nils GustafssonSCSiân Culley
Siân CulleyGAGeorge Ashdown
George AshdownDODylan M. Owen
Dylan M. Owen
PPPedro Pereira
Pedro Pereira
Ricardo HenriquesDespite significant progress, high-speed live-cell super-resolution studies remain limited to specialized optical setups, generally requiring intense phototoxic illumination. Here, we describe a new analytical approach, super-resolution radial fluctuations (SRRF), provided as a fast graphics processing unit-enabled ImageJ plugin. In the most challenging data sets for super-resolution, such as those obtained in low-illumination live-cell imaging with GFP, we show that SRRF is generally capable of achieving resolutions better than 150 nm. Meanwhile, for data sets similar to those obtained in PALM or STORM imaging, SRRF achieves resolutions approaching those of standard single-molecule localization analysis. The broad applicability of SRRF and its performance at low signal-to-noise ratios allows super-resolution using modern widefield, confocal or TIRF microscopes with illumination orders of magnitude lower than methods such as PALM, STORM or STED. We demonstrate this by super-resolution live-cell imaging over timescales ranging from minutes to hours.
27 September 2019 in Nature Methods
Journal Article
JTJervis Vermal Thevathasan
Jervis Vermal Thevathasan
MKMaurice Kahnwald
Maurice KahnwaldKCKonstanty Cieśliński
Konstanty Cieśliński
PHPhilipp Hoess
Philipp HoessSPSudheer Kumar Peneti
Sudheer Kumar Peneti
JRJonas Ries
Jonas Ries +14
19 February 2018 in Nature Methods
Journal Article
SCSiân Culley
Siân CulleyDADavid Albrecht
David AlbrechtCJCaron Jacobs
Caron JacobsPPPedro Pereira
Pedro PereiraCLChristophe Leterrier
Christophe LeterrierRicardo Henriques +1
17 August 2015 in Nature Communications
Journal Article
JMJoão M. Monteiro
João M. MonteiroPFPedro B. Fernandes
Pedro B. FernandesFVFilipa Vaz
Filipa VazAPAna R. Pereira
Ana R. PereiraATAndreia C. Tavares
Andreia C. TavaresMPMariana G. Pinho
Mariana G. Pinho +7
Staphylococcus aureus is an aggressive pathogen and a model organism to study cell division in sequential orthogonal planes in spherical bacteria. However, the small size of staphylococcal cells has impaired analysis of changes in morphology during the cell cycle. Here we use super-resolution microscopy and determine that S. aureus cells are not spherical throughout the cell cycle, but elongate during specific time windows, through peptidoglycan synthesis and remodelling. Both peptidoglycan hydrolysis and turgor pressure are required during division for reshaping the flat division septum into a curved surface. In this process, the septum generates less than one hemisphere of each daughter cell, a trait we show is common to other cocci. Therefore, cell surface scars of previous divisions do not divide the cells in quadrants, generating asymmetry in the daughter cells. Our results introduce a need to reassess the models for division plane selection in cocci.
13 October 2010 in Proceedings of the National Academy of Sciences
Journal Article
MAMagda L. Atilano
Magda L. AtilanoPPPedro Pereira
Pedro PereiraJYJames Yates
James YatesPRPatricia Reed
Patricia ReedHVHelena Veiga
Helena VeigaSFSérgio R. Filipe
Sérgio R. Filipe +1
The cell wall of Staphylococcus aureus is characterized by an extremely high degree of cross-linking within its peptidoglycan (PGN). Penicillin-binding protein 4 (PBP4) is required for the synthesis of this highly cross-linked peptidoglycan. We found that wall teichoic acids, glycopolymers attached to the peptidoglycan and important for virulence in Gram-positive bacteria, act as temporal and spatial regulators of PGN metabolism, controlling the level of cross-linking by regulating PBP4 localization. PBP4 normally localizes at the division septum, but in the absence of wall teichoic acids synthesis, it becomes dispersed throughout the entire cell membrane and is unable to function normally. As a consequence, the peptidoglycan of TagO null mutants, impaired in wall teichoic acid biosynthesis, has a decreased degree of cross-linking, which renders it more susceptible to the action of lysozyme, an enzyme produced by different host organisms as an initial defense against bacterial infection.
21 March 2012 in Science Translational Medicine
Journal Article
CTChristopher M. Tan
Christopher M. Tan
ATAlex G. Therien
Alex G. Therien
JLJun Lu
Jun Lu
SLSang H. Lee
Sang H. Lee
ACAlexandre Caron
Alexandre Caron
TRTerry Roemer
Terry Roemer +32
Methicillin-resistant Staphylococcus aureus infection can be treated effectively by combining a β-lactam antibiotic with a drug that targets FtsZ.
About Me
Researcher at the Bacterial Cell Biology Lab of MOSTMICRO at ITQB-NOVA. Pedro holds a BSc on Applied Chemistry – Biotechnology (2006) from FCT-NOVA, a Master in biotechnology (2007) and a PhD degree in Cell Biology (2013) both from ITQB-NOVA. From 2014 to 2019 Pedro Pereira was a Research Associate at the Quantitative Imaging and Nanobiophysics lab of MRC-Laboratory for Molecular Biology (MRC-LMCB) from University College London (UCL) and an invited lecturer at the European Molecular Biology Laboratory (EMBL-Heidelberg). From 2017 to 2019 Pedro was also a University partner at the Francis Crick Institute. His research focuses on microbiology and host-pathogen interaction using state of the art technology (e.g. super-resolution microscopy). From a scientific perspective, Pedro has made contributions in the fields of S. aureus microbiology, host-pathogen interaction and hardware/software technological innovations for microscopy. Besides his dedication and commitment to research Pedro has a keen interest in embracing the different aspects of knowledge creation and sharing, as such he is actively engaged in science outreach initiatives, teaching and has served in several decision boards in the institutions he worked in.