The current investigation was conducted to assess the melissopalynological, physicochemical, and biochemical properties, antimicrobial and antioxidant activities as well as total phenolic and total flavonoid contents of 794 Sidr honey samples collected from the Saudi market that had been imported from 12 different countries. Testing Sidr honey from different countries showed different levels of growth suppression observed against five drug resistant bacterial strains. The pathogenic strains were Staphylococcus aureus, Streptococcus mutans, Klebsiella pneumoniae, Escherichia coli and Pseudomonas aeruginosa. The antimicrobial activity showed growth suppression levels which varied according to the origin of the honey. The comparative study of Sidr honeys revealed a strong correlation between total polyphenol and flavonoid contents and significant radical scavenging activities in particular Egyptian and Saudi Arabian honeys. The melissopalynological and physicochemical properties of different Sidr honeys complied with the recommendations of the WHO Codex Alimentarius, the European Union standards for honey quality, and the Gulf Technical Regulation on honey (GSO 147:2008-Standards Store-GCC Standardization Organization). It was concluded that Sidr honey from different geographical areas has the capacity to suppress the growth of pathogenic bacteria and perform significant radical scavenging activities.