Platelets undergo significant morphological transformations during activation influenced by chemical compounds such as zinc and milrinone. Manual classification methods are subjective, time-consuming, and prone to error. This research aimed to apply and evaluate the efficacy of different pre-trained deep learning Convolution Neural Networks, used for automated binary (treated vs. untreated) and multiclass classification (control vs. zinc vs. milrinone vs. combination) of platelet morphological changes induced by these solvents, addressing the limitations of manual classification methods. A dataset of 176 microscopic platelet images from Hull York Medical School was processed, comprising control (n=43), zinc-treated (n=45), milrinone-treated (n=40), and combination treatment (n=48) samples. Ten pre-trained CNN architectures were evaluated across 166 individual training iterations for both binary and multiclass classification tasks. Progressive optimisation strategies included contrast-limited adaptive histogram equalisation (CLAHE) enhancement, selective layer unfreezing, cross-validation, and hyperparameter tuning. For binary classification, Xception achieved optimal balanced performance (81% accuracy, 76% precision, 74% recall) with proper inter-class discrimination, despite higher numerical metrics (91% accuracy) showing classification bias. For multiclass classification, InceptionV3 and Xception demonstrated superior effectiveness with 72% accuracy. This study demonstrated that Xception and InceptionV3 architectures effectively capture subtle morphological features distinguishing treated platelets, offering reproducible alternatives to subjective manual methods.

Platelets undergo significant morphological transformations during activation influenced by chemical compounds such as zinc and milrinone. Manual classification methods are subjective, time-consuming, and prone to error. This research aimed to apply and evaluate the efficacy of different pre-trained deep learning Convolution Neural Networks, used for automated binary (treated vs untreated) and multiclass classification (control vs zinc vs milrinone vs combination) of platelet morphological changes induced by these solvents, addressing the limitations of manual classification methods. A dataset of 176 microscopic platelet images from Hull York Medical School was processed, comprising control (n = 43), zinc-treated (n = 45), milrinone-treated (n = 40), and combination treatment (n = 48) samples. Ten pre-trained CNN architectures were evaluated across 166 individual training iterations for both binary and multiclass classification tasks. Progressive optimisation strategies included contrast-limited adaptive histogram equalisation (CLAHE) enhancement, selective layer unfreezing, cross-validation, and hyperparameter tuning. For binary classification, Xception achieved optimal balanced performance (81% accuracy, 76% precision, 74% recall) with proper inter-class discrimination, despite higher numerical metrics (91% accuracy) showing classification bias. For multiclass classification, InceptionV3 and Xception demonstrated superior effectiveness with 72% accuracy. This study demonstrated that Xception and InceptionV3 architectures effectively capture subtle morphological features distinguishing treated platelets, offering reproducible alternatives to subjective manual methods.