As an alternative to conventional lighting systems, light emitting diode (LED) has been demonstrated to be an artificial flexible lighting source for commercial micropropagation. The objective of this study was to determine the effects of different LED light quality on in vitro shoot proliferation and growth of Vanilla planifolia. To evaluate shoot proliferation, axillary bud cuttings (3 to 5 mm in diameter) of V. planifolia were used as explants and cultivated on Murashige and Skoog basal medium supplemented with 9.55 µM of 6-benzylaminopurine. To evaluate in vitro growth, unrooted shoots (2 cm in length) were used as explants cultivated on Murashige and Skoog basal medium without plant growth regulators. All explants were exposed to a 16 h photoperiod for 60 days under five different lights: fluorescent lamp, white LED, blue LED, red LED and blue plus red LED mixtures (Blue + Red = 1:1). The results indicated a clear increase in the number of shoots per explant under Fluorescent Lamp, White LED and Blue+Red LED light; these treatments produced more than 10 shoots. Shoot length was more than 3 cm in cultures under Blue, Red and Blue+Red (1:1) LEDs, and less than 3 cm in Fluorescent Lamp and White LEDs. Our results also showed that fresh weight, dry weight and dry matter were greatest in shoots under Blue+Red LED light. For shoot growth, plant height, number of leaves, number and length of roots, fresh weight, dry weight, dry matter and chlorophyll content were greater under Fluorescent Lamp and White LED. In conclusion, White or Blue+Red LED light may be used as an alternative light source for shoot proliferation, while White LED may be used for growth in vitro. These results demonstrate the effectiveness of light qualities using LEDs for micropropagation of V. planifolia. Key words: Light quality, micropropagation, orchid, chlorophyll.

During the dry processing of coffee, beans are classified by size, shape, weight, and color, resulting in the commercial preparation requested by the client, who can be of American, European, or other descent. The preparations consist of a combination of bean sizes and shapes, with a specified maximum number of defects. The objective of this work was to evaluate the effect of the shape and size of the bean of the three main Catimors grown in Mexico, i.e., Colombia, Costa Rica, and Oro Azteca, on the cupping quality attributes of the beverage. The results showed that there was no significant difference between the cupping quality parameters of the Colombia and Costa Rica varieties, whereas Oro Azteca exhibited a final score significantly lower compared to those varieties. For beans of size 13/64 (Z13), significant differences were found between bean forms (flat, peaberry, and mixed) of the Colombia variety. Flat (FL) beans scored almost seven points higher in the final score compared to peaberries (PE). In coffee beans of size Z14 or Z15, mixed (MI) samples scored equal or significantly higher than FL samples in the three varieties. Small beans (Z13 and Z14) of samples not separated by shape (PE and FL together) obtained equal or significantly higher scores in the cup quality attributes of the beverage compared to bigger beans. It was demonstrated that the shape and size of the coffee bean significantly affect the cup quality parameters depending upon the variety.