The Shitake mushroom (Lentinula edodes) is the second most-consumed mushroom in the world; in Colombia, it is cultivated and commercialized on a small scale in some supermarkets. Little is known about the precedence, nutritional and medicinal properties of Shiitake produced in Colombia. In this study, four shiitake isolates were grown in Colombia (LEUCO1, LEUCO2, LEUCO3, and LEUCO4) were sequenced in their ITS genes and evaluated for the production of three medicinal metabolites, eritadenine, ergotioneine and β-glucans (1,3-1, 6), using submerged culture. Genetic analysis revealed that all the isolates were close and related to the Japanese strain Cr62. LEUCO1 and LEUCO2 showed a distance of 0.000, as well as LEUCO3 and LEUCO4. All four isolates produced erythadenin in a range of 26.3-8.6 mg / L, with the best performance of LEUCO1 at 26.3 mg / L (p <0.05). Ergotioneine was produced with similar statistical yields in all the isolates with an average of 0.7 mg / g of dry weight biomass (DW). Β-glucans (1.3-1.6) were produced with yields of 5.6 - 3.8% of DW biomass, with the best values ​​for LEUCO2 and the lowest for LEUCO4 (p <0.05). In conclusion, we identified low genetic diversity in the four isolates, corresponding to two haplotypes with minimal genetic difference between them, related to the Japanese strain Cr62, indicating that Colombian farmers cultivate almost the same strains of shitake. Secondary metabolites, eritadenine, β-glucans and ergotioneine were found in promising yields useful for the pharmaceutical and food industries. More studies should be conducted to improve the yield of shitake metabolites through new growing conditions for industrial production and to find metabolic pathways and related genes.

Methane–hydrogen–air mixtures present a viable alternative to conventional fuels, reducing CO₂ emissions while maintaining high energy density. This study numerically investigates their combustion characteristics in millimeter-scale reactors, focusing on flame stabilisation and combustion dynamics in confined spaces. A species transport model with volumetric reactions incorporated a detailed kinetic mechanism with 16 species and 41 reactions. The simulations employed a laminar flow model, second-order upwind discretisation, and SIMPLE algorithm for pressure–velocity coupling. The key parameters analysed include equivalence ratio, hydrogen volume fraction, inlet velocity, and gas pressure and their impact on fuel conversion efficiency and heat release was evaluated. The results indicate that hydrogen enrichment enhances flame stability and combustion efficiency, with optimal performance over 40% hydrogen content. Additionally, increased outlet pressure raises flame temperature by 15%, while larger reactor diameters reduce heat losses, improving combustion efficiency by 20%. Emissions of CO decrease significantly at higher hydrogen fractions, demonstrating the potential for cleaner combustion. These findings support the integration of methane–hydrogen mixtures into sustainable energy systems, providing insights for designing efficient, low-emission micro-combustors.

This work aims to select a photoreactor flow configuration and operational conditions that maximize the Photocatalytic Space-time Yield in a photoelectrocatalytic reactor to degrade Reactive Red 239 textile dye. A numerical study by Computational Fluid Dynamics (CFD) was carried out to model the phenomena of momentum and species transport and surface reaction kinetics. The photoreactor flow configuration was selected between axial (AF) and tangential (TF) inlet and outlet flow, and it was found that the TF configuration generated a higher Space-time Yield ($STY$) than the AF geometry in both laminar and turbulent regimes due to the formation of a helical movement of the fluid, which generates velocity in the circumferential and axial directions. In contrast, the AF geometry generates a purely axial flow. In addition, to maximize the Photocatalytic Space-time Yield ($PSTY$), it is necessary to use solar radiation as an external radiation source when the flow is turbulent. In conclusion, the $PSTY$ can be maximized up to a value of 45 g/day-kW at an inlet velocity of 0.2 m/s (inlet Reynolds of 2830), solar radiation for external illumination, and internal illumination by UV-LEDs of 14 W/m², using a photoreactor based on tangent inlet and outlet flow.

Hydrogen is emerging as a key energy vector for the transition toward renewable and sustainable energy sources. However, its safe and efficient storage remains a significant technical challenge in terms of cost, safety, and performance. In this study, we aimed to address the kinetic limitations of Mg by synthesizing catalyzed Mg@Ni systems using commercially available micrometric magnesium particles (~26 µm), which were decorated via electroless nickel plating under both aqueous and anhydrous conditions. Morphological and compositional characterization was carried out using SEM, EDS, and XRD. The resulting materials were evaluated through Temperature-Programmed Desorption (TPD), DSC, and isothermal hydrogen absorption/desorption kinetics. Reversibility over multiple absorption–desorption cycles was also investigated. The synthesized Mg@NiB system shows a reduction of 37 °C in the hydrogen release activation temperature at atmospheric pressure and a decrease of 167.3 °C under high vacuum conditions (4.5 × 10⁻⁷ MPa), in addition to a reversible hydrogen absorption/desorption capacity of 3.5 ± 0.09 wt.%. Additionally, the apparent activation energy for hydrogen desorption was lower (161.7 ± 21.7 kJ/mol) than that of hydrogenated commercial pure magnesium and was comparable to that of milling MgH₂ systems. This research is expected to contribute to the development of efficient and low-cost processing routes for large-scale Mg catalysis.