Plant growth-promoting rhizobacteria (PGPR) not only promote growth and heavy metal uptake by plants but are promising biosorbents for heavy metals remediation. However, there exist arguments over whether extracellular adsorption (biosorption) or intracellular accumulation (bioaccumulation) play dominant roles in Cd(II) adsorption. Therefore, three cadmium-resistant PGPR, Cupriavidus necator GX_5, Sphingomonas sp. GX_15, and Curtobacterium sp. GX_31 were used to study bioaccumulation and biosorption mechanisms under different initial Cd(II) concentrations, using batch adsorption experiments, desorption experiments, scanning electron microscopy coupled with energy dispersive X-ray (SEM-EDX) spectroscopy, transmission electron microscopy (TEM), and Fourier-transform infrared (FTIR) spectroscopy. In this study, with the increase of the initial Cd(II) concentrations, the removal efficiency of strains decreased and the adsorption capacity improved. The highest Cd(II) removal efficiency values were 25.05%, 53.88%, and 86.06% for GX_5, GX_15, and GX_31 with 20 mg l⁻¹ of Cd(II), while the maximum adsorption capacity values were 7.97, 17.13, and 26.43 mg g⁻¹ of GX_5, GX_15, and GX_31 with 100 mg l⁻¹ of Cd(II). Meanwhile, the removal efficiency and adsorption capacity could be ordered as GX_31 > GX_15 > GX_5. The dominant adsorption mechanism for GX_5 was bioaccumulation (50.66–60.38%), while the dominant mechanisms for GX_15 and GX_31 were biosorptions (60.29–64.89% and 75.93–79.45%, respectively). The bioaccumulation and biosorption mechanisms were verified by SEM-EDX, TEM and FTIR spectroscopy. These investigations could provide a more comprehensive understanding of metal-bacteria sorption reactions as well as practical application in remediation of heavy metals.

Plant growth-promoting rhizobacteria (PGPR) have been extensively investigated in combination remediation with plants in heavy metal contaminated soil. However, being biosorbent, few studies of live and dead cells of PGPR have been undertaken. Meanwhile, the application of live or dead biomass for the removal of heavy metals continues to be debated. Therefore, this study uses living and non-living biosorbents of Cupriavidus necator GX_5, Sphingomonas sp. GX_15, and Curtobacterium sp. GX_31 to compare their Cd(II) adsorption capacities by SEM-EDX, FTIR, and adsorption experiments. In the present study, whether the cells were living or dead and whatever the initial Cd(II) concentration was, removal efficiency and adsorption capacity can be arranged as GX_31 > GX_15 > GX_5 (p < 0.05). However, removal efficiency in live and dead biosorbents was quite different and it greatly affected by the initial Cd(II) concentrations. The dead cells exhibited a higher adsorption capacity than the live cells of GX_31. Nevertheless, for GX_5 and GX_15, the loading capacity of the non-living biomass was stronger than that of the living biomass at 20 mg L⁻¹ of Cd(II), but the capacity was similar at 100 mg L⁻¹ of Cd(II). Minor changes of spectra were found after autoclaving and it seemed that more functional groups of the dead biosorbent were involved in Cd(II) binding by FTIR analysis, which also illustrated that the hydroxyl, amino, amide, and carboxyl groups played an important role in complexation with Cd(II). Based on these findings, we concluded that the dead cells were more potent for Cd(II) remediation, especially for GX_31.

Biosorbents have been extensively studied for heavy metal adsorption due to their advantages of low cost and high efficiency. In the study, the living and non-living biomass of Cupriavidus necator GX_5 previously isolated were evaluated for their adsorption capacity and/or removal efficiency for Cd (II) through batch experiments, SEM and FT-IR investigations. The maximum removal efficiency rates for the live and dead biomass were 60.51% and 78.53%, respectively, at an optimum pH of 6, a dosage of 1 g/L and an initial Cd (II) concentration of 5 mg/L. The pseudo-second-order kinetic model was more suitable for fitting the experimental data, indicating that the rate-limiting step might be chemisorption. The Freundlich isotherm model fit better than the Langmuir isotherm model, implying that the adsorption process of both biosorbents was heterogeneous. FT-IR observation reflected that various functional groups were involved in Cd (II) adsorption: –OH, –NH, C=O, C–O and C–C groups for the living biomass and –OH, –NH, C–H, C = O, C–N and N–H groups for the dead biomass. Our results imply that non-living biosorbents have a higher capacity and stronger strength for absorbing Cd (II) than living biomass. Therefore, we suggest that dead GX_5 is a promising adsorbent and can be used in Cd (II)-contaminated environments.