This study aimed at the production of biosurfactants from yeasts under acidic conditions using residual soybean oil as a carbon source, as well as the biosurfactant produced in the solubilization of metals in sewage sludge. The yeast Meyerozyma guilliermondii was considered the best producer in both pH 4.0 and 2.0; therefore, the product obtained by this yeast was characterized by Fourier transform infrared (FT-IR) and ¹H nuclear magnetic resonance (NMR) spectroscopy. Moreover, it was applied in metal removal assays in anaerobic sewage sludge. The spectra obtained in FT-IR suggested that M. guilliermondii's biosurfactant had a similar structure to glycolipids from the sophorolipid class, and it was confirmed by ¹H NMR spectroscopy. In the bioleaching assays, the application of biosurfactant (2%) produced by M. guilliermondii with pH adjusted to 2.0 was able to solubilize 15.9% of cadmium from the sewage sludge.

Saxitoxins are a class of toxins produced by at least two groups of evolutionarily distant organisms (cyanobacteria and dinoflagellates). While the toxicity of these toxins is relatively well characterized, to date little is known about their drivers and ecological functions, especially in lower latitude tropical and subtropical freshwater ecosystems. In the present study, we aimed to obtain a better understanding of the main drivers of saxitoxin concentrations in aquatic environments. We investigated the relationships among saxitoxin concentrations in a mesotrophic subtropical reservoir dominated by the cyanobacteria Cylindrospermopsis raciborskii with physical, chemical and biological water variables. The highest saxitoxin concentrations were 0.20 μg·L⁻¹, which occurred in the samples with the highest densities of C. raciborskii (maximum of 4.3 × 10⁴ org·mL⁻¹) and the highest concentration of dissolved nutrients (nitrate from 0.2 to 0.8 μg·L⁻¹, ortophosphate from 0.3 to 8.5 μg·L⁻¹). These correlations were confirmed by statistical analyses. However, the highest saxitoxin relative concentrations (per trichome) were associated with lower C. raciborskii densities, suggesting that saxitoxin production or the selection of saxitoxin-producing strains was associated with the adaptation of this species to conditions of stress. Our results indicate that C. raciborskii toxin yields vary depending on the enrichment conditions having potential implications for reservoir management.