The classification of germinated pole sitao (Vigna unguiculata (L.) Walp.) seeds is important in seed germination tests. The automation of this process has been explored for different grain and legume seeds but is only limited to binary classification. This study aimed to develop a classifier system that can recognize three classes: normal, abnormal, and ungerminated. SSD MobileNet and Faster R-CNN models were trained to perform the classification. Both were trained using 1500 images of germinated seeds at fifth- and eighth-day observations. Each class had 500 images. The trained models were evaluated using 150 images per class. The SSD MobileNet model had an accuracy of 0.79 while the Faster R-CNN model had an accuracy of 0.75. The results showed that the average accuracies for the classes were significantly different from one another based on one-way ANOVA at a 95% confidence level with an F-critical value of 3.0159. The SSD MobileNet model outperformed the Faster R-CNN model in classifying pole sitao seeds, with improved precision in identifying abnormal and ungerminated seeds on the fifth day and normal and ungerminated seeds on the eighth day. The results confirm the potential of the SSD MobileNet model as a more reliable classifier in germination tests.

Efficient feed management is essential in aquaculture, especially for bottom-feeding species such as shrimp that require feed delivery at the tank bottom. Most commercial automated feeders are designed for surface-feeding fish and are unsuitable for benthic organisms, leading to feed waste and uneven distribution. This study developed and evaluated an automatic bottom feeder capable of dispensing sinking pellets directly to the substrate. The system integrated a 3D-printed auger for precise feed metering and a closed-type centrifugal impeller positioned at the water surface to achieve radial dispersion of feed. An Arduino Uno microcontroller operated the impeller speed (285.98–586.85 rpm), feed mass (95.23–285.68 g), and dispersion time (2–8 s). A Box–Behnken response surface methodology was used to analyze the influence of these parameters on the mean radius spread of feed, supported by image-based uniformity assessment using OpenCV. Results identified impeller speed as the most significant factor (p = 0.010), with optimal dispersion observed at moderate speeds and longer spread durations. The system demonstrated reliable mechanical performance and precise control, providing a novel, programmable solution for uniform feed delivery in shrimp aquaculture and a promising foundation for scalable, automated bottom-feeding technologies.

Deoxyribonucleic acid (DNA)-based biosensors are rapid, cost-effective, and portable devices for monitoring crop pathogens. However, their on-field operations rely on a laboratory-bound heating block, which controls temperature during sample preparation. This study aimed to develop a field-deployable heating block to assist in the DNA hybridization protocol of DNA-based biosensors. It should maintain $95 °\mathrm{C}$, $55 °\mathrm{C}$, and $20 °\mathrm{C}$ for 5, 10, and 5 min, respectively. It had aluminum bars, positive thermal coefficient ceramic heaters, a Peltier thermoelectric module, and DS18B20 thermistors, serving twelve 0.2 mL polymerase chain reaction (PCR) tubes. An Arduino microcontroller employing a proportional–integral–derivative (PID) algorithm with a solid-state relay was utilized. Machine performance for distilled water-filled PCR tubes showed a maximum $10 °\mathrm{C}$ thermal variation. The machine maintained $(96.00\pm 0.97) °\mathrm{C}$, $(55.15\pm 2.17) °\mathrm{C}$, and $(17.75\pm 0.71) °\mathrm{C}$ with root mean square errors (RMSEs) of $1.40 °\mathrm{C}$, $2.18 °\mathrm{C}$, and $2.36 °\mathrm{C}$, respectively. The average thermal rates were $(0.16\pm 0.11) °\mathrm{C}/\mathrm{s}$, $(0.29\pm 0.11) °\mathrm{C}/\mathrm{s}$, and $(0.14\pm 0.07) °\mathrm{C}/\mathrm{s}$ from ambient to $95 °\mathrm{C}$, $95 °\mathrm{C}$ to $55 °\mathrm{C}$, and $55 °\mathrm{C}$ to $20 °\mathrm{C}$, respectively. Overall, the low standard deviations and RMSEs demonstrate thermostable results and robust temperature control.