To investigate the relevance of zinc in host-pathogen interactions, we have constructedSalmonella entericamutant strains in which theznuAgene, which encodes the periplasmic component of the ZnuABC high-affinity Zn²⁺transporter, was deleted. This mutation does not alter the ability ofSalmonellato grow in rich media but drastically reduces its ability to multiply in media deprived of zinc. In agreement with this phenotype, ZnuA accumulates only in bacteria cultivated in environments poor in zinc. In spite of the nearly millimolar intracellular concentration of zinc, we have found thatznuAis highly expressed in intracellular salmonellae recovered either from cultivated cells or from the spleens of infected mice. We have also observed thatznuAmutants are impaired in their ability to grow in Caco-2 epithelial cells and that bacteria starved for zinc display decreased ability to multiply in phagocytes. A dramatic reduction in the pathogenicity of theznuAmutants was observed inSalmonella-susceptible (BALB/c) orSalmonella-resistant (DBA-2) mice infected intraperitoneally or orally. This study shows that the amount of free metals available for bacterial growth within the infected animal is limited, despite the apparent elevated concentration of free metals within cells and in plasma and suggests thatSalmonellaexploits the ZnuABC zinc transporter to maximize zinc availability in such conditions. These results shed new light on the complex functions of zinc in vertebrate and bacterial physiology and pave the way for a better comprehension of pathogenic mechanisms inSalmonellainfections.