Cluster computing combines the resources of multiple computers as they act like a single high-performance computer. In this study, a computer cluster consisting of Lustre distributed file system with one cluster server based on Slurm resource management system and thirteen calculation nodes were built by using available and inert computers that have different processors. Different bioinformatics algorithms were run using different data sets in the cluster, and the performance of the clusters was evaluated with the amount of time the computing cluster spent to finish the jobs.