The coalescent methods for species tree reconstruction are increasingly popular because they can accommodate coalescence and multilocus data sets. Herein, we present STRAW, a web server that offers workflows for reconstruction of phylogenies of species using three species tree methods—MP-EST, STAR and NJst. The input data are a collection of rooted gene trees (for STAR and MP-EST methods) or unrooted gene trees (for NJst). The output includes the estimated species tree, modified Robinson-Foulds distances between gene trees and the estimated species tree and visualization of trees to compare gene trees with the estimated species tree. The web sever is available at http://bioinformatics.publichealth.uga.edu/SpeciesTreeAnalysis/.

Significance: The α C– β 4 loop is a conserved structural motif present in all eukaryotic protein kinases. This study focuses on epidermal growth factor receptor (EGFR), a receptor tyrosine kinase that harbors many recurrent insertion mutations in the α C– β 4 loop. We show that EGFR activity and drug sensitivity are strongly dependent on the nature, size, and location of mutations in the loop and that even subtle variations in the loop have a dramatic effect on kinase dynamics and free-energy landscape. These findings inform ongoing drug-discovery efforts on EGFR kinases and provide a structural framework for investigating the role of the understudied α C– β 4 loop in kinase regulation, dynamics, and evolution.