A leaf spot of rice (Oryza sativa L.) was observed in a survey conducted in different districts of Punjab, Pakistan, from August to October, 2012. Symptoms appeared as brown spots on the leaf blade and on the small emerging leaves. Primary symptoms were brown small, round-ovoid spots with a chlorotic halo, evenly distributed over the leaf. The size of spots varies from 10mm – 1 cm. Over time, spots merged and became large. Infected leaves were collected and washed in running water thoroughly for 5-10 min. Small pieces (0.5-2 cm) of leaf tissue were then surface disinfected with 2% NaOCl for 2 min, rinsed three to four times in sterile distilled water (SDW), blotted dry on sterile filter paper and placed on potato dextrose agar (PDA) plates. PDA plates were incubated at 28 0C for 5-7 days. A single spore fungal isolate was maintained on PDA for further investigations. Morphological and microscopic characteristics and phylogenetic analysis were used for the identification for the fungal isolate. On PDA, fun...