Serial section transmission electron microscopy (ssTEM) reveals biological information at a scale of nanometer and plays an important role in the ultrastructural analysis. However, due to the imperfect preparation of biological samples, ssTEM images are usually degraded with various artifacts that greatly challenge the subsequent analysis and visualization. In this paper, we introduce a unified deep learning framework for ssTEM image restoration which addresses three main types of artifacts, i.e., Support Film Folds (SFF), Staining Precipitates (SP), and Missing Sections (MS). To achieve this goal, we first model the appearance of SFF and SP artifacts by conducting comprehensive analyses on the statistics of real degraded images, relying on which we can then simulate a large number of paired images (degraded/artifacts-free) for training a deep restoration network. Then, we design a coarse-to-fine restoration network consisting of three modules, i.e., interpolation, correction, and fusion. The interpolation module exploits the adjacent artifacts-free images for an initial restoration, while the correction module resorts to the degraded image itself to rectify the artifacts. Finally, the fusion module jointly utilizes the above two results to further improve the restoration fidelity. Experimental results on both synthetic and real test data validate the significantly improved performance of our proposed framework over existing solutions, in terms of both image restoration fidelity and neuron segmentation accuracy. To the best of our knowledge, this is the first unified deep learning framework for ssTEM image restoration from different types of artifacts. Code is available at https://github.com/sydeng99/ssTEM-restoration.

Electron microscopy (EM) image denoising is critical for visualization and subsequent analysis. Despite the remarkable achievements of deep learning-based non-blind denoising methods, their performance drops significantly when domain shifts exist between the training and testing data. To address this issue, unpaired blind denoising methods have been proposed. However, these methods heavily rely on image-to-image translation and neglect the inherent characteristics of EM images, limiting their overall denoising performance. In this paper, we propose the first unsupervised domain adaptive EM image denoising method, which is grounded in the observation that EM images from similar samples share common content characteristics. Specifically, we first disentangle the content representations and the noise components from noisy images and establish a shared domain-agnostic content space via domain alignment to bridge the synthetic images (source domain) and the real images (target domain). To ensure precise domain alignment, we further incorporate domain regularization by enforcing that: the pseudo-noisy images, reconstructed using both content representations and noise components, accurately capture the characteristics of the noisy images from which the noise components originate, all while maintaining semantic consistency with the noisy images from which the content representations originate. To guarantee lossless representation decomposition and image reconstruction, we introduce disentanglement-reconstruction invertible networks. Finally, the reconstructed pseudo-noisy images, paired with their corresponding clean counterparts, serve as valuable training data for the denoising network. Extensive experiments on synthetic and real EM datasets demonstrate the superiority of our method in terms of image restoration quality and downstream neuron segmentation accuracy. Our code is publicly available at https://github.com/sydeng99/DADn.