Indicators can monitor ecological environment changes and help maintain ecological balance. Bioindicators are divided into animal, plant, and microbial indicators, of which animal and plant indicators have previously been the most researched, but microbial indicators have drawn attention recently owing to their high sensitivity to the environment and their potential for use in monitoring environmental changes. To date, reviews of studies of animals and plants as indicator species have frequently been conducted, but reviews of research on microorganisms as indicator species have been rare. In this review, we summarize and analyze studies using microorganisms as indicator species in a variety of ecosystems, such as forests, deserts, aquatic and plateau ecosystems, and artificial ecosystems, which are contained in wetlands, farmlands, and mining ecosystems. This review provides useful information for the further use of microorganisms as indicators to reflect the changes in different environmental ecosystems.

In recent years, invasive alien plants (IAPs) have caused serious ecological disasters and economic losses in China. This study combined three IAP species richness-related indices (species richness of IAPs, first records of IAPs, and the relative species richness of IAPs), as well as indices reflecting distribution and dispersal patterns (average similarity coefficient of IAPs) and invasiveness (average risk score of IAPs), to conduct an integrated regional-invasion risk assessment based on the principal component analysis (PCA) method. Partial least-squares (PLS) regression was conducted to explore the explanatory power of 12 environmental and anthropogenic factors on different invasion indices. The results indicated that coastal provinces and Yunnan had high IAP introduction risk, as well as high synthetic-risk scores. The dispersal of IAPs in mid-latitude provinces should be particularly prevented. For species richness of IAPs, more environmental factors with variable importance for the project (VIP) values higher than 1 were retained in the optimal model, reflecting the importance of environmental filtering on IAPs. Visitors were the most important predictor for first records of IAPs. Compared to species richness (R² = 79.5%), first records were difficult to predict (R² = 60.4%) and were influenced by anthropogenic factors. There was spatial distribution congruence of various families of IAPs. Generally, the correlations of the residuals of species richness were still significant, with 0.421 (p < 0.05) as the lowest Pearson correlation coefficient, which indicated that external factors could not fully explain the spatial distribution congruence. These findings could enrich the relevant research on IAP invasion mechanisms and provide suggestions for regional IAP detection and response.

Anorthoa Berio, 1980 is a genus within the subfamily Hadeninae . This genus has a close relationship with the rama species complex of the genus Harutaeographa. It comprises three species groups: the munda-group, the angustipennis-group and the rubrocinerea-group. The species Anorthoa changi Ronkay & Ronkay, 2001 from Taiwan Island belongs to the rubrocinerea-group. A new species related to Anorthoa changi Ronkay & Ronkay, 2001, is described from Wuyishan National Park, China. The new species can be distinguished from its sister species by its larger size and some differences in the genitalia. Now, the number of species in the genus Anorthoa is increased to eleven.

A new species of the genus Synochoneura Obraztsov, 1955, S. wuyishana sp. nov. is described and illustrated from Wuyishan National Park in Fujian Province of China. The new species resembles S. sapana, but differs in wing pattern and female genitalia characters. Illustrations of adults and genitalia are provided. Additionally, a key to the Chinese Synochoneura species based on morphology is presented. The type specimens are deposited in the Department of Entomology, College of Plant Protection, South China Agricultural University, Guangzhou.

Phormia regina (Meigen, 1826) (Diptera: Calliphoridae), a necrophagous blow fly of significant forensic value, plays a crucial role in postmortem interval (PMI) estimation. The intra-puparial period of this species constitutes approximately half of its entire immature developmental duration. Therefore, precise aging of intra-puparial specimens is essential to improve the accuracy of PMI estimation. This study was performed at a constant 25 °C, examining intra-puparial morphology, histological sections, and body weight across different developmental stages. Additionally, full-length transcriptome profiling was conducted via a hybrid sequencing strategy combining PacBio single-molecule real-time (SMRT) sequencing and Illumina short-read next-generation sequencing (NGS). Morphological studies revealed the intra-puparial morphological characteristics, the reconstruction process of tissues and organs, and the continuous body weight loss during the intra-puparial period. Transcriptomic research identified a total of 425,349 full-length non-chimeric (FLNC) sequences. After redundancy removal, 84,852 transcript sequences were obtained, of which 46,325 transcripts were annotated. Using day-0 puparia (D0) as the control, differentially expressed gene (DEG) analysis was performed on samples from subsequent developmental stages (D1–D5), identifying 4242, 7964, 9509, 10,526, and 10,011 DEGs, respectively. Results from GO enrichment and KEGG pathway analyses provided reasonable explanations for the behavioral traits of different developmental stages. Eight candidate genes for intra-puparial development were screened, most of which were highly expressed at different time points during the intra-puparial period. Quantitative real-time PCR (qRT-PCR) results showed consistent gene expression trends with the RNA-seq data, confirming the reliability of the RNA-seq results. This study provides key morphological and molecular markers for P. regina in forensic PMI estimation.