Molecular imprinted solid-phase extraction is the technique that uses molecular imprinted polymer as the sorbent in solid phase extraction. Molecular imprinted solid-phase extraction is effective and efficient for the extraction process and cleaning as compared with solid phase extraction (SPE) without molecular imprinted polymer. The complexity of variables in molecular imprinted solid-phase extraction arise as problems in the analysis, therefore it is necessary to optimize the extraction conditions of molecular imprinted solid-phase extraction. To achieve the sorption equilibrium and achieve the shortest time, certain parameters such as contact time, ion strength of sample, pH of sample, amount of sorbent, sample flow rate, addition of salt and buffer solution, washing solvent, elution solvent, and loading solvent need to be optimized. The selection of suitable properties and quantities of each factor greatly affect the formation of appropriate interactions between the sorbent and analytes. Percentage recovery is also influenced by formation of the appropriate bonds, sample flow rates, extraction time, salt addition, and sorbent mass. Therefore, in the future, molecular imprinted solid-phase extraction optimization has to consider and adjust various factors reviewed in this paper to form appropriate interactions between the absorbent and target molecules which have an impact on the optimal results.