Evidence of fruit rot disease was observed in twelve bell pepper (Capsicum annuum L.) fields located in the Chakwal district (32°56′00′′N, 72°51′30′′ E) of Pakistan during the period June 2016 to 2017. Disease incidence was estimated to be between 15% and 24%. Yield loss in affected fields was estimated as 11%. Symptoms of disease were seen as water soaked, discolored necrotic lesions predominantly around the calyx. There were also symptoms of internal rot as seeds and the inner fruit surface were covered with white to light gray fungal growth. In the case of severe disease symptoms, fungal growth was also observed externally at the calyx end. The disease progressed as a soft decay with leakage. Diseased fruits were cut into 5 mm² pieces, surface-disinfested with 1% NaOCl for 2 minutes, rinsed thrice with sterile distilled water, and incubated on Potato Dextrose Agar (PDA) at 25±2°C for 3-4 days under continuous fluorescent light. Eighteen isolates of similar morphology were purified using the hyphal tip ...