Tomato (Solanum lycopersicum L.) is one of the important vegetable crop widely cultivated across the world. In Pakistan, tomato is being cultivated on an area of 159,000 ha with an annual production of 2,901,000 tons (FAO. 2014). In April-May 2014, wilted tomato plants were observed with yellow leaves and yellowish to brown stem coloration that eventually dried to brown were observed in Punjab, Pakistan. Symptomatic plants were easily uprooted and black sclerotial bodies were observed in the lower stems of the infected plants with roughly shredded appearance. Root and stem portions from symptomatic plants were cut into small pieces, surface sterilized with 1% sodium hypochlorite for 3 to 5 min followed by a rinse with sterile distilled water, blotting on filter paper and were transferred onto potato dextrose agar (PDA) amended with chloramphenicol (250mg/L). PDA plates were incubated at 25 ± 2°C for 7 days. Fungal colonies isolated on PDA medium were initially hyaline but later became grey with plenty of ...