Pakistan is among the top 15 citrus producing countries in the world, with an area of 199,000 ha and 19.7 MT annual citrus production. In 2014, a survey was conducted in three major citrus growing areas (Sargodha, Khushab and Bhalwal) of Punjab province, where 94% of Pakistan’s citrus production is concentrated (Tahir, 2014). Severe dieback was observed in orchards, resulting in 40% yield losses. Dead twigs of Citrus reticulata cv. Kinnow produced small black pustules. Other symptoms included leaf chlorosis that eventually affected the whole plant. Samples were collected from twigs of symptomatic plants with more than 40% canopy infection from different orchards. Short segments encompassing lesion margins were excised, surface-sterilized with 70% ethanol, placed on potato dextrose agar (PDA), and incubated at 30 ± 1°C for 3 days with 12 h alternate light and dark cycle. Two different fungal morphotypes were recovered in a 2:1 frequency ratio. To obtain pure cultures, hyphal tips were transferred onto fres...