Soft Rot Pectobacteriaceae including Pectobacterium and Dickeya species are responsible for causing potato blackleg in the field and tuber soft rots in storage conditions reducing the yield and quality of the tubers (Adeolu et al. 2016). Since several decades, D. dianthicola has been reported as a plant pathogenic bacterium in USA, Australia, many European countries and in Morocco as well (Oulghazi et al. 2016). In January 2017, potato plants exhibiting blackleg symptoms were observed in local potato fields in the Okara district of Punjab, Pakistan. Initially, the stem bases of infected plants blackened and then rotted with vascular discoloration. Bacteria were isolated from the symptomatic part of the stem showing blackleg symptoms on the nutrient agar medium followed by streaking of the isolates on the specific crystal violet pectate (CVP) medium (Hélias et al. 2012). After 3 days on CVP at 28°C, pectinolytic bacteria showed the formation of pits around the bacterial colonies. To identify the pectinolyt...