The early maize (Zea mays) seed undergoes several developmental stages after double fertilization to become fully differentiated within a short period of time, but the genetic control of this highly dynamic and complex developmental process remains largely unknown. Here, we report a high temporal-resolution investigation of transcriptomes using 31 samples collected at an interval of 4 or 6 h within the first six days of seed development. These time-course transcriptomes were clearly separated into four distinct groups corresponding to the stages of double fertilization, coenocyte formation, cellularization, and differentiation. A total of 22,790 expressed genes including 1415 transcription factors (TFs) were detected in early stages of maize seed development. In particular, 1093 genes including 110 TFs were specifically expressed in the seed and displayed high temporal specificity by expressing only in particular period of early seed development. There were 160, 22, 112, and 569 seed-specific genes predominantly expressed in the first 16 h after pollination, coenocyte formation, cellularization, and differentiation stage, respectively. In addition, network analysis predicted 31,256 interactions among 1317 TFs and 14,540 genes. The high temporal-resolution transcriptome atlas reported here provides an important resource for future functional study to unravel the genetic control of seed development.

This is a research report on the improvement of the humanized elements of unmanned system control station design. For this purpose, the first step is to set the ergonomics elements in control station design as user requirements, which are divided into three levels: physiological needs, psychological needs, and ergonomic needs. Besides, by further proposing analytical method based on user requirements priorities of AHP, the authors of the present report employ HOQ combining user requirements and their corresponding ergonomic design characteristics to solve relative design problems through TRIZ theory related methods. Finally, the feasibility and effectiveness of the above techniques and methods are verified by use of examples.