Cadmium (Cd) accumulation in rice grain poses a serious threat to human health. While several transport systems have been reported, the complexity of rice Cd transport and accumulation indicates the necessity of identifying additional genes, especially those that are responsible for Cd accumulation divergence between indica and japonica rice subspecies. Here, we show that a gene, OsCd1, belonging to the major facilitator superfamily is involved in root Cd uptake and contributes to grain accumulation in rice. Natural variation in OsCd1 with a missense mutation Val449Asp is responsible for the divergence of rice grain Cd accumulation between indica and japonica. Near-isogenic line tests confirm that the indica variety carrying the japonica allele OsCd1^V449 can reduce the grain Cd accumulation. Thus, the japonica allele OsCd1^V449 may be useful for reducing grain Cd accumulation of indica rice cultivars through breeding.

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Arsenic (As) contamination in paddy soils is a global problem, threatening rice production and food safety. Hyperaccumulator plants have garnered significant attention for their potential to remove pollutants from contaminated soil. However, no natural hyperaccumulators have been found for the phytoremediation of As-contaminated paddy soils under flooding conditions. One promising strategy is to genetically engineer Oryza sativa (rice) to hyperaccumulate As for effective phytoremediation of paddy soil. A key challenge remains in increasing metal accumulation without compromising tolerance. Here, PvACR3 from the As hyperaccumulator Pteris vittata was introduced under the control of a rice root-specific promoter pLsi1 to create high-As-accumulating and tolerant transgenic remediation rice. The remediation rice strains exhibited robust growth, with shoot As concentration reaching up to 451–557 ​mg/kg in a hydroponic experiment with 20 ​μM NaAsO₂ treatment, and 45.9–80.3 ​mg/kg in pot experiments with moderately As-contaminated paddy soils. Compared to wild-type rice, the pLsi1::PvACR3 transgenic rice removed 23.5 times more As from the same paddy soils. By harvesting rice shoots before grain filling, the soil pore water As was almost completely depleted, and the acid-soluble and reducible fractions of As were significantly reduced. This study presents the first transgenic remediation rice characterized by high As accumulation, tolerance, and adaptability to paddy soils under flooding conditions for effective phytoremediation.