In the historical mysteries and present pandemic situation, the use of citrus fruits makes it rise high among other fruits. Citrus has a significant role in dietary and medicinal purposes from time immemorial and widely acknowledged for its therapeutic properties. Citrus megaloxycarpa Lush. is an unspecified sibling of the citrus family. The present work highlights the use of the cryptic species of North-East India with its efficient biochemical, antimicrobial, anticancerous properties activity. The aim of this study was to characterize analyzed various biochemical, antioxidant, anticancerous parameters. The peel and pulp extract of ripening large citrus sample indicated as P(L₁) and Pu(L₂) respectively and unripe small peel and pulp extracts was indicated as P(S₁) and Pu(S₂) respectively. The extract of the Pu(L₂) has the highest total soluble protein whereas the extract of P(L₁) demonstrated high in carbohydrate concentration. On quantifying the free amino acid value, the extract of P(L₂) showed higher in quantification. The total DPPH scavenging activity was compared for the extracts, where the extract of Pu(L₂) exhibit high IC50 value. The present work concluded the use of Citrus megaloxycarpa Lush. peel and pulp extracts to be an efficient therapeutic drug.