Vitamin D, obtained from diet or synthesized internally as cholecalciferol and ergocalciferol, influences bodily functions through its most active metabolite and the vitamin D receptor. Recent research has uncovered multiple roles for vitamin D in the central nervous system, impacting neural development and maturation, regulating the dopaminergic system, and controlling the synthesis of neural growth factors. This review thoroughly examines these connections and investigates the consequences of vitamin D deficiency in neurological disorders, particularly neurodegenerative diseases. The potential benefits of vitamin D supplementation in alleviating symptoms of these diseases are evaluated alongside a discussion of the controversial findings from previous intervention studies. The importance of interpreting these results cautiously is emphasised. Furthermore, the article proposes that additional randomised and well-designed trials are essential for gaining a deeper understanding of the potential therapeutic advantages of vitamin D supplementation for neurological disorders. Ultimately, this review highlights the critical role of vitamin D in neurological well-being and highlights the need for further research to enhance our understanding of its function in the brain.

Accurate quantification of wound closure in cell migration assays is crucial yet challenging. Still, existing methods often underperform due to omitting cell detection within the wound area, resulting in biased outcomes. We developed the CSMA standalone and ImageJ-compatible tool, which utilizes advanced image processing techniques, including contrast enhancement, edge detection, and morphological operations, to precisely identify and quantify cells in the wound region. CSMA offers user-friendly features and adjustable parameters to accommodate different imaging conditions, ensuring robust performance across diverse experimental setups. Validation against conventional tools confirms CSMA’s superior ability to delineate wound boundaries and provide accurate estimations of area and width at every time point. As applied to SW480-ADH colon cancer cells treated with various compounds, CSMA proves valuable in biomedical research. CSMA represents a significant advancement in wound healing assay analysis, providing researchers with a simple and reliable tool for studying cell migration dynamics with enhanced precision and reproducibility. CSMA is available as a standalone and ImageJ-compatible tool with its source code at https://github.com/AminaSagymbayeva/CSMA_WoundHealing.

Accurate quantification of wound closure in cell migration assays is crucial yet challenging. Still, existing methods often underperform due to omitting cell detection within the wound area, resulting in biased outcomes. To overcome this limitation, we developed the CSMA plugin for ImageJ. CSMA utilizes advanced image processing techniques, including contrast enhancement, edge detection, and morphological operations, to precisely identify and quantify cells in the wound region. The plugin offers user-friendly features and adjustable parameters to accommodate different imaging conditions, ensuring robust performance across diverse experimental setups. Validation against conventional tools confirms CSMA’s superior ability to delineate wound boundaries and provide accurate estimations of area and width at every time point. As applied to SW480-ADH colon cancer cells treated with various compounds, CSMA proves valuable in biomedical research. It represents a significant advancement in wound healing assay analysis, providing researchers with a simple and reliable tool for studying cell migration dynamics with enhanced precision and reproducibility. CSMA is available as an ImageJ plugin and source code at https://github.com/AminaSagymbayeva/CSMA_WoundHealing .