⁴⁴Sc is a promising β ⁺-emitter for molecular imaging with intermediate half-life of 4 h. Due to the chemical similarity of Sc³⁺ to the Lu³⁺ and Y³⁺ cations, ⁴⁴Sc-DOTA bioconjugates are expected to demonstrate similar properties in vivo as the ¹⁷⁷Lu- and ⁹⁰Y-bioconjugates, what is important in planning the radionuclide therapy. ⁴⁴Sc can be obtained from the ⁴⁴ Ti/⁴⁴Sc generator. An alternative method for ⁴⁴Sc production can be the irradiation of ⁴⁴Ca target at small cyclotrons. The aim of our work was to optimize the parameters of ⁴⁴CaCO₃ irradiation and to develop a simple procedure for ⁴⁴Sc separation from the calcium target. For optimization study, ⁴⁴CaCO₃ targets were irradiated by protons in the energy range of 5.6–17.5 MeV with 9 MeV being found to be the best energy for ⁴⁴Ca irradiations. A simple and fast separation procedure of ⁴⁴Sc from calcium target was developed using chelating resin Chelex 100. DOTATATE conjugate was successfully radiolabelled with high yield at elevated temperature using the produced ⁴⁴Sc. While ⁴⁴CaCO₃ is relatively expensive, the cost of ⁴⁴Sc-DOTATATE production can be reduced by target recovery. Due to low proton energy required to produce GBq activity level of ⁴⁴Sc, the availability of ⁴⁴Sc radioisotope could be enhanced to open new opportunities for applications in medical imaging.