Experiments were conducted to test several methods for estimating low temperature thresholds for seed germination. Temperature responses of nine weeds common in annual agroecosystems were assessed in temperature gradient experiments. Species included summer annuals (Amaranthus albus, A. palmeri, Digitaria sanguinalis, Echinochloa crus‐galli, Portulaca oleracea, and Setaria glauca), winter annuals (Hirschfeldia incana and Sonchus oleraceus), and Conyza canadensis, which is classified as a summer or winter annual. The temperature below which development ceases (T_base) was estimated as the x‐intercept of four conventional germination rate indices regressed on temperature, by repeated probit analysis, and by a mathematical approach. An overall T_base estimate for each species was the average across indices weighted by the reciprocal of the variance associated with the estimate. Germination rates increased linearly with temperature between 15 °C and 30 °C for all species. Consistent estimates of T_base were obtained for most species using several indices. The most statistically robust and biologically relevant method was the reciprocal time to median germination, which can also be used to estimate other biologically meaningful parameters. The mean T_base for summer annuals (13.8 °C) was higher than that for winter annuals (8.3 °C). The two germination response characteristics, T_base and slope (rate), influence a species’ germination behaviour in the field since the germination inhibiting effects of a high T_base may be offset by the germination promoting effects of a rapid germination response to temperature. Estimates of T_base may be incorporated into predictive thermal time models to assist weed control practitioners in making management decisions.

Giant reed is one of the most widespread invasive species in riparian habitats in California and other coastal states of the United States. This species is thought to spread primarily asexually by flood dispersal of stem and rhizome pieces; viable seeds have not been found in the United States. Research was conducted to quantify genetic variation in giant reed along the Santa Ana River in California and to investigate the pattern of distribution of variation along this watershed. Populations at least 3.2 km apart were collected along the length of the Santa Ana River from the headwaters to the Pacific Ocean. One additional population from a different watershed was collected to serve as an out-group. Genetic analyses were conducted using both starch gel electrophoresis for isozyme analysis and random amplified polymorphic DNA (RAPD) analysis. Both isozyme and RAPD analyses revealed levels of genetic diversity comparable with those in the literature for clonal species, suggesting that asexual reproduction is the primary means of spread of giant reed. Most phenotypes were spread along the Santa Ana River, which is expected if water is the primary means of spread of vegetative propagules. Among the unique phenotypes found, two isozyme phenotypes and one RAPD phenotype were dominant and were found spread along the river, which may indicate greater fitness or competitive superiority to the other phenotypes that were less common. The dominant phenotypes were also found in the out-group population, possibly because of spread by humans. Because spread occurs mainly asexually, management efforts should focus on preventing establishment and spread of vegetative propagules. A moderate level of genetic diversity also suggests that biological control of this weed could be successful.

Seasonality of clonal propagation in giant reed - Volume 49 Issue 6 - Joseph G. Decruyenaere, Jodie S. Holt

Abutilon theophrasti is one of the worst agricultural weeds in North America, yet it has not reached that status in California in the 80 yr since it was first reported. The research reported here examined the distribution and modeled climatic requirements of A. theophrasti to determine whether it is likely to spread more widely in the state. Herbaria records and weed literature were surveyed to determine the historical occurrences of A. theophrasti in the state; current distribution was assessed through surveys sent to University of California personnel in each county. Combined results showed 42 counties out of 58 with A. theophrasti present historically or currently. A plot of the cumulative number of counties containing A. theophrasti by decade fit a logistic equation. The maximum rate of spread of this species occurred in 1962 and it is likely that its final distribution by county in California is leveling off and not likely to increase further. The climate-matching/mapping software CLIMEX® was used with observed and estimated parameters of environmental requirements of A. theophrasti to model its current distribution from India through China to Japan. The same model parameters were then used to map its potential distribution in California. Areas where A. theophrasti has been reported were predicted by CLIMEX to be poorly suited for its growth and development without added soil moisture in the form of irrigation. It appears that the Mediterranean climate is a deterrent to the integration of A. theophrasti into California. The climate-matching approach provided a biologically reasonable assessment of potential distribution of A. theophrasti in California. The approach also allowed assessment of the effects of common agricultural practices on potential distribution given the environmental requirements and limitations of A. theophrasti.