Microalgae-based bulk production, especially for biofuel, is not yet economically feasible. One way to improve financial performance of such systems is to maximise value through a biorefinery approach (e.g. co-production streams, waste minimisation). This can be implemented via extraction of valuable intracellular compounds upon cell disruption; however both low-cost, energy efficient, and scalable cell disruption techniques, and green chemicals are required. Waste heat is a readily available resource in most production plants, and can be used to power a hydrothermal pre-treatment (HTP) step to disrupt the cells. In this study, we use HTP (120–180 °C, 5–30 min) to disrupt Chlorella sp. cell walls. We perform a subsequent water-miscible solvent and/or urea extraction (UX) to solubilise and recover proteins. The maximum extraction efficiency of 62% was achieved after HTP at 180 °C for 10 min. Regardless of the reaction time, 120 °C was found to be ineffective for protein extraction. The efficiency can be further increased to 84% if a filter-washing step with acetone and urea is implemented. Most of the recovered proteins are between 8 to 14 kDa across all temperature ranges, suggesting that extraction efficiency depended both on partial protein hydrolysis and on accessibility to solvent (i.e. cell wall degradation, solid dispersion, membrane solubilisation). The most significant amino acid losses on a per mole basis were lysine (22%) and arginine (14%). The production of flexible and high-value protein-based products via HTP-UX may enable a circular and sustainable production step for microalgal biorefineries, while improving the downstream fuel processes through nitrogen heteroatom reduction.

Wave energy has the potential to play an important role in the UK's electricity mix in the coming years and it is important to understand the interactions of wave energy converters with the environment before considering them viable alternatives for other technologies. The aim of this study was to identify the environmental impacts of the deployment of the Oyster wave energy converter to the EMEC test site at Orkney, UK over its lifetime across three general categories: resource use, human health and ecological consequences. A full life cycle assessment was performed on two different models of the Oyster wave energy converter: Oyster 1 and Oyster 800. It was found that the latter is a fitting upgrade for its predecessor as it has lower environmental impacts in all categories; however, the high infrastructural needs of the Oyster technology makes its environmental performance worse than most other wave energy converters. Key sustainability indicators for energy converters include carbon footprint and energy payback period, and these were found to be 79 and 57 gCO₂ eq/kWh and 45 and 42 months for the Oyster 1 and Oyster 800, respectively. Although these are significantly higher than most estimates for other types of renewable energy converter, the carbon impacts are still significantly lower than for conventional fossil-fuelled power generation.