Bis-ADC complexes cis-[Pd{C(NHC₆H₄NH₂)N(H)R}₂]Cl₂ (R = Xyl 4a, Cy 4b, C₆H₄-4-F 4c) and cis-[Pt{C(NHC₆H₄NH₂)N(H)R}₂]Cl₂ (R = Xyl 5a, Cy 5b, C₆H₄-4-F 5c) were synthesized via the metal-mediated coupling of two isocyanide ligands in cis-[MCl₂(CNR)₂] (M = Pd, Pt; R = Xyl, Cy, C₆H₄-4-F) and 1,2-diaminobenzene. New compounds 4c and 5a–c were characterized by HR ESI⁺-MS, IR, and ¹H, ¹³C{¹H} and ¹⁹⁵Pt{¹H} NMR spectroscopy; the structures of 4a and 5a were elucidated by single-crystal X-ray diffraction. The stability of the ADC complexes in aqueous media (5 mM NaCl) was monitored by UV absorption spectroscopy, HR ESI⁺ mass spectrometry, and ¹⁹⁵Pt{¹H} NMR spectroscopy (for 5a). Molar conductivity measurements in MeOH (Λ_M = 167–173 Ω⁻¹ mol⁻¹ cm²) indicate that, in this solvent, the ADC complexes exist as dicationic species of [A][Q]₂ type. The ADC complexes binding to CT DNA was investigated by means of spectroscopic and hydrodynamic techniques including UV absorption and circular dichroism spectroscopy, fluorescence spectroscopy, low-gradient viscometry, flow birefringence, and AFM imaging. As a result, complexes 4a and 5a were shown to bind double-stranded DNA predominantly via the formation of monofunctional adducts in the major groove of the macromolecule. Binding of the ADC complexes also provokes the formation of a large number of intermolecular DNA–DNA contacts in solution. The antiproliferative activity of all prepared ADC complexes 4a–c and 5a–c was evaluated in vitro against three human carcinoma cell lines (HT-29, MDA-MB-231, and MCF-7) and two non-tumorigenic cell lines (L929 and RC-124) and compared to that of cisplatin. Among the compounds studied, complexes 4a and 5a appeared to be the most active species with IC₅₀ values in MCF-7 cells of about 10 μM.

The synthesis of 2-pyridyltellurenyl bromide via Br₂ oxidative cleavage of the Te–Te bond of dipyridylditelluride is reported. Single-crystal X-ray diffraction analysis of 2-pyridyltellurenyl bromide demonstrated that the Te atom of 2-pyridyltellurenyl bromide was involved in four different noncovalent contacts: Te⋯Te interactions, two Te⋯Br ChB, and one Te⋯N ChB contact forming 3D supramolecular symmetrical framework. In contrast to 2-pyridylselenenyl halides, the Te congener does not react with nitriles furnishing cyclization products. 2-Pyridylselenenyl chloride was demonstrated to easily form the corresponding adduct with benzonitrile. The cyclization product was studied by the single-crystal X-ray diffraction analysis, which revealed that in contrast to earlier studied cationic 1,2,4-selenadiazoles, here we observed that the adduct with benzonitrile formed supramolecular dimers via Se⋯Se interactions in the solid state, which were never observed before for 1,2,4-selenadiazoles.