In December 2019, the first cases of the novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) were identified in the city of Wuhan, China. Since then, it has spread worldwide with new mutations being reported. The aim of the present study was to monitor the changes in genetic diversity and track non-synonymous substitutions (dN) that could be implicated in the fitness of SARS-CoV-2 and its spread in different regions between December 2019 and November 2020. We analyzed 2213 complete genomes from six geographical regions worldwide, which were downloaded from GenBank and GISAID databases. Although SARS-CoV-2 presented low genetic diversity, there has been an increase over time, with the presence of several hotspot mutations throughout its genome. We identified seven frequent mutations that resulted in dN substitutions. Two of them, C14408T>P323L and A23403G>D614G, located in the nsp12 and Spike protein, respectively, emerged early in the pandemic and showed a considerable increase in frequency over time. Two other mutations, A1163T>I120F in nsp2 and G22992A>S477N in the Spike protein, emerged recently and have spread in Oceania and Europe. There were associations of P323L, D614G, R203K and G204R substitutions with disease severity. Continuous molecular surveillance of SARS-CoV-2 will be necessary to detect and describe the transmission dynamics of new variants of the virus with clinical relevance. This information is important to improve programs to control the virus.

Bio-based fertilisers (BBFs) are gaining attention as sustainable alternatives to mineral fertilisers due to their potential for nutrient recovery, reduced environmental emissions, and improved soil health. However, their broader adoption is hindered by regulatory uncertainty, quality inconsistencies, and methodological variability in assessing their environmental impacts. This study can reveal about the body of research on bio-based fertilisers (BBFs), using a hybrid methodology that combines bibliometric and content analysis. A total of 247 publications from 2001 to 2024 were reviewed to identify research trends, environmental concerns, and assessment approaches. Results show a sharp increase in BBF-related publications after 2016, driven primarily by European and North American research, with growing focus on life cycle assessment (LCA) and nutrient recovery. The in-depth analysis of the ten most cited LCA and non-LCA studies highlights key methodological differences: non-LCA studies frequently rely on empirical fieldwork and generate primary data, whereas LCA studies typically synthesise secondary data to provide broader system-level insights. Despite this complementarity, the lack of methodological harmonisation poses a barrier to consistent comparison and interpretation. The findings highlight the need for a unified, standardised assessment framework to reliably evaluate the environmental performance of BBFs and support their effective implementation within circular and sustainable agricultural systems.

Mediterranean agroecosystems are highly vulnerable to climate change, soil degradation, and declining soil organic carbon (SOC), threatening long-term agricultural sustainability. Carbon farming and regenerative agriculture have emerged as complementary approaches to restore soil functionality while contributing to climate change mitigation. This review synthesizes peer-reviewed literature published between 2015 and 2025 to assess the agronomic effectiveness of key regenerative and carbon farming practices in Mediterranean systems. A structured bibliographic analysis using Scopus and Web of Science evaluated practices influencing SOC dynamics, erosion control, water regulation, and associated ecosystem services. Evidence indicates that the introduction of cover crops in the crop rotation and reduced or no-tillage are the most consistently effective practices for enhancing SOC stocks, particularly when combined with organic amendments and diversified rotations. Crop diversification, intercropping, and agroforestry further support SOC accumulation and erosion control, especially in perennial systems such as vineyards and olive orchards. Organic inputs stimulate microbial-mediated carbon stabilization, while regenerative grazing contributes to nutrient cycling under context-specific conditions. Across practices, integrated management consistently delivers greater and more stable benefits than single interventions. Regenerative agriculture thus provides a systems-based foundation for carbon farming in Mediterranean agroecosystems. Long-term field experiments and improved monitoring frameworks remain essential to quantify carbon persistence and support policy implementation.

Loess–palaeosol sequences (LPSs) are continuous records of palaeoenvironmental and palaeoclimatic conditions during the Quaternary. This study includes 17 LPS located in the NE of the Iberian Peninsula, irregularly distributed, associated with different river basins: the Ebro Basin, the Mora Basin, and the Ter sub-basin. The soils developed on these loess deposits present a loam–sandy texture, coarser than the typical loess, ochre in colour, with variable thickness (1–12 m), calcareous composition (20–45% CaCO₃ eq.), very low or null organic matter (OM), and basic pH. These deposits have been classified as desert LPS, whose pedogenesis is mainly associated with the redistribution of calcium carbonate and, in some cases, gypsum. Several methodologies have been applied to determine their mineralogical, physical, and chemical characteristics and date them by luminescence techniques. In addition, some relevant pedofeatures (porosity, CaCO₃, gypsum, etc.) have been characterised in detail. The aims of the present study have been to know the pedogenic development of the LPS by defining the main soil-forming factors that have affected them in order to associate these factors with the characteristic palaeoclimatic and palaeoenvironmental conditions reported in this area over time and to improve the understanding of soil evolution.