Background: Canine impaction complicates treatment and prolongs duration, requiring precise localization. CBCT is the gold standard for diagnosis and assessment. However, it involves high radiation exposure and cost. This study aimed to evaluate the effectiveness of a combined biomechanical approach for orthodontic traction of impacted maxillary canines (IMCs) and to determine whether intraoral scans (STL files) could replace a final CBCT in assessing canine repositioning. Methods: The sample included 10 patients (7 males and 3 females) with 13 severely displaced IMCs, treated with a protocol combining Invisalign^® aligners, elastics, mini-implants, and sectional wires. In all, 9 IMC were palatally impacted, while 4 were buccally impacted. A representative clinical case is presented to illustrate the biomechanics used in one of the complex cases. Canine movement was evaluated at the cusp and apex through two methods: overlay of pre- and post-treatment CBCTs, and overlay of initial and final STL scans onto the initial CBCT. Results: A Class I canine relationship was successfully achieved in all patients. No statistically significant differences were found between the two measurement methods (p > 0.05). Conclusions: Orthodontic traction of IMC, especially in complex cases, can be achieved using aligners, elastics, mini-implants, and sectional wires. Once the canine crown has erupted and is clinically visible, STL scans overlaid with the initial CBCT can accurately assess the final position of the crown and root. This allows clinicians to avoid a second CBCT in selected cases, reducing patient radiation exposure while maintaining diagnostic accuracy.

Background: In the last decade, infections caused by Candida species have increased. Although C. albicans remains the most predominant species, fungal infections caused by non-albicans Candida (NAC) species have also been rising. This study aimed to determine which Candida spp. are most frequently associated with oral candidiasis. Methods: In accordance with PRISMA guidelines, a literature search was conducted in the PubMed, Cochrane Library, and ScienceDirect databases. The search used the keyword combination “candida spp” AND “oral candidiasis” AND “oral isolates” and included articles published between 2013 and 31 October 2025. Results: A total of 658 articles were identified, of which 24 met the inclusion criteria. Across these studies, 12,750 isolates were reported. C. albicans was the most prevalent species, accounting for 81.7% of all isolates. NAC species were detected at lower frequencies, including C. tropicalis (7.2%), C. glabrata (4.5%), C. krusei (4.1%), C. parapsilosis (1.0%), C. dubliniensis (0.8%), C. kefyr (0.2%), C. guilliermondii (0.1%), C. lusitaniae (0.1%), and other rare or unidentified species (0.2%). The increasing prevalence of Candida infections is associated with a growing population of immunocompromised individuals, and treatment remains challenging due to rising antifungal resistance. Conclusions: Although C. albicans remains the most prevalent, the appearance of NAC species is gradually increasing. With the increase of Candida spp. resistant to conventional antifungal agents and with the competitive or synergistic interaction between Candida spp., it is necessary to develop new therapeutic approaches.

Background: Limosilactobacillus reuteri (L. reuteri), present in the oral and intestinal microbiota, can colonize the oral cavity through breastfeeding and dairy intake, promoting oral health by balancing the microbiota, inhibiting pathogens, and modulating immune responses. This study aimed to evaluate the preventive role and therapeutic potential of L. reuteri in pediatric oral health. Methods: A literature review was conducted using PubMed, Wiley Library, and the Cochrane Library, supplemented by manual screening, according to PRISMA guidelines and covering the period from January 2011 to 31 December 2024. Results: From 835 records identified, 12 studies met the inclusion criteria. Data shows that L. reuteri strains produce antimicrobial substances that disrupt biofilms and inhibit Streptococcus mutans and other lactobacilli, leading to increased oral pH and improved periodontal indices. The effectiveness of probiotics was found to be strain-specific and transient, with continuous intake and adequate oral hygiene enhancing their ability to colonize the oral cavity. Conclusions: Probiotics show significant potential as therapeutic interventions for controlling cariogenic bacteria and supporting gum health in children. Through mechanisms including bacterial co-aggregation, competitive exclusion, antimicrobial compound synthesis, and immune modulation, probiotics may effectively reduce the risk of tooth decay and gum disease. Their effectiveness depends on the strain, regular intake, proper dosing, good oral hygiene, and suitable delivery, which enhance oral colonization and clinical benefits.

Background: Artificial intelligence (AI) integrated with cone-beam computed tomography (CBCT) has rapidly advanced the diagnostic capability of orthodontics, particularly for quantifying external root resorption (ERR). High-risk scenarios such as bilateral maxillary canine impaction require objective tools to guide treatment decisions and prevent irreversible damage. Objectives: To evaluate the diagnostic accuracy and clinical applicability of AI-assisted CBCT for orthodontically induced ERR, and to demonstrate its value in a complex clinical case where decision-making regarding canine traction versus extraction required precise risk quantification and definition of biological limits. Methods: A systematic review following PRISMA 2020 guidelines was conducted in PubMed, ScienceDirect, and Cochrane Library (2015–September 2025). Eligible studies applied AI-enhanced CBCT to assess ERR in orthodontic patients. Additionally, a clinical case with bilaterally impacted maxillary canines was evaluated using CBCT with automated AI segmentation and manual refinement to quantify root volume changes and determine prognostic thresholds for treatment modification. Results: Nine studies met the inclusion criteria. AI-based imaging, predominantly convolutional neural networks, showed high diagnostic accuracy (up to 94%), improving reproducibility and reducing operator dependency. In the clinical case, volumetric monitoring showed rapid progression of ERR in the lateral incisors (LI) associated with a persistent unfavorable 3D spatial relationship between the canines and incisor roots, despite controlled distal traction with skeletal anchorage, leading to a timely change in the treatment plan and extraction of the severely compromised incisors with substitution by the canines. AI-generated data provided objective evidence supporting safer decision-making and prevented further structural deterioration. Conclusions: AI-enhanced CBCT enables early, objective, and quantifiable ERR assessment, strengthening prognosis-based decisions in orthodontics. Findings of this review and the clinical case highlight the translational relevance of AI for managing high-risk cases, such as maxillary canine impaction with extensive LI resorption, supporting future predictive AI models for safer canine traction.

Background/Objectives: The oral microbiome plays a central role in maintaining oral health from early life, with childhood representing a critical period for its establishment and long-term stability. While many environmental factors influence this dynamic microbial ecosystem, diet is distinct in being both universal and highly modifiable. This systematic review aims to evaluate and synthesize current evidence regarding the impact and mechanisms of distinct dietary habits, food matrices, and nutritional components on the composition, diversity, and ecological resilience of the pediatric oral microbiome. Methods: A literature review aligned with PRISMA guidelines was conducted via digital searches on PubMed, ScienceDirect, and Cochrane databases (January 2015–December 2025). Search strategies combined MeSH terms and keywords targeting “Microbiota”, “Mouth”, “Child”, “Diet”, and “Oral health”. Results: From 1068 records identified, 16 relevant articles met the inclusion criteria. Dietary habits may influence taxonomic and functional profiles. Frequent consumption of sugar-sweetened beverages, sucrose-rich sodas, and sweet treats induces notable dysbiosis and enriches acidogenic/aciduric taxa. Conversely, protective food matrices, including probiotic-fortified dairy products, polyol-based sugar-free chewing gums (xylitol and maltitol), bovine milk, and bioactive-rich agents like green tea, actively suppress cariogenic pathways (specifically Streptococcus mutans) and support commensal, health-associated genera without disrupting overall microbial structures. Conclusions: Diet represents an important modifiable factor shaping the pediatric oral microbiome, capable of either driving dysbiosis or reinforcing symbiosis. Cultivating a microbiome-informed dietary approach early in childhood supports a resilient microbial architecture, offering a non-invasive, public health framework for long-term oral and systemic disease prevention.

Objectives: This systematic review compares the clinical efficacy and radiographic outcomes of membrane-based regeneration, bone grafting, and platelet concentrates in cavities resulting from apicoectomy, assessing healing rates and the quality of newly formed bone. Methods: Following PRISMA 2020 guidelines, a literature search was performed in PubMed, Cochrane Library, ScienceDirect, Wiley, and Scopus databases for articles published between January 2000 and December 2025. Eligibility criteria were defined using the PICOS framework, and included studies were evaluated based on clinical and radiographic parameters with a minimum follow-up of six months. Results: Eighteen clinical studies were included. Findings were heterogeneous and predominantly of low to very low certainty. Although some studies suggested potential benefits of regenerative adjuncts in complex defects, such as through-and-through and apicomarginal lesions, a substantial proportion found no statistically significant advantage over conventional apical surgery. Smaller lesions (<3 mm) generally responded favorably to conventional surgery, whereas larger defects may benefit from adjunctive regenerative approaches in selected clinical situations. Conclusions: Overall, the available evidence suggests a potential benefit of combining physical support (biomaterials) and biological stimulation (platelet-rich fibrin) in regenerative apical surgery. However, findings remain heterogeneous and should be interpreted cautiously. Technique selection should consider defect morphology, lesion characteristics, and available resources. Further standardized and methodologically robust studies are required to strengthen the current evidence base.