Essential oils of aromatic plants represent an alternative to classical pest control with synthetic chemicals. They are especially promising for the alternative control of stored product pest insects. Here, we tested behavioral and electrophysiological responses of the stored product pest Tribolium confusum, to the essential oil of a Brazilian indigenous plant, Varronia globosa, collected in the Caatinga ecosystem. We analyzed the essential oil by GC-MS, tested the effects of the entire oil and its major components on the behavior of individual beetles in a four-way olfactometer, and investigated responses to these stimuli in electroantennogram recordings (EAG). We could identify 25 constituents in the essential oil of V. globosa, with anethole, caryophyllene and spathulenole as main components. The oil and its main component anethole had repellent effects already at low doses, whereas caryophyllene had only a repellent effect at a high dose. In addition, the essential oil abolished the attractive effect of the T. confusum aggregation pheromone. EAG recordings revealed dose-dependent responses to the individual components and increasing responses to the blend and even more to the entire oil. Our study reveals the potential of anethole and the essential oil of V. globosa in the management of stored product pests.

The present study aimed to assess the effects of the essential oil of Croton pulegiodorus Baill on eight populations of Sitophilus zeamais Motschulsky with different patterns of susceptibility to synthetic insecticides. Populations of S. zeamais were obtained from Sete Lagoas-MG, Jacarezinho-PR, Bom Conselho-PE, Garanhuns-PE, Jupi-PE, Lajedo-PE, São João-PE and Serra Talhada-PE. To estimate the lethal concentrations (LC50 and LC90) of oil for each population, fumigation tests were performed. The susceptibility of S. zeamais to the essential oil varied among populations. Garanhuns and Bom Conselho was considered the susceptibility patterns, presenting the lowest LC50 (3.40 μL L-1 of air) and LC90 (9.60 μL L-1 of air) for the essential oil, respectively. The population from Jupi exhibited the highest LC50 (14.49 μL L-1 of air) and LC90 (19.60 μL L-1 of air) for C. pulegiodorus. The resistance ratio ranged from 1.84 for the São João to 4.26 for the Jupi population. Thus, the essential oil of C. pulegiodorus showed fumigant activity, causing mortality in all S. zeamais populations used.