Pseudomonas aeruginosa is a pathogen of critical priority importance according to the WHO. Due to its multi-resistance and expression of various virulence factors, it is the causal agent of severe healthcare-acquired infections (HAIs). Effective strategies to control infections caused by P. aeruginosa must include early and specific detection of the pathogen for early and timely antibiotic prescription. The need to develop a specific and reproducible diagnostic technique is urgent, which must often be more sensitive and faster than current clinical diagnostic methods. In this study, we implement and standardize the loop-mediated isothermal amplification (LAMP) technique, coupled with the reverse line blot hybridization (RLBH) technique for the detection of P. aeruginosa. A set of primers and probes was designed to amplify a specific region of the P. aeruginosa 16s rRNA gene. The sensitivity of the LAMP-RLBH method was 3 × 10⁻⁴ ng/μL, 1000 times more sensitive than the PCR and LAMP technique (this work), with a sensitivity of 3 × 10⁻³ ng/μL. The LAMP-RLBH and LAMP techniques showed specific amplification and no cross-reaction with members of the ESKAPE group and other Pseudomonas species. The present investigation provides a technique that can be easily performed in less time, achieving a faster and more reliable alternative compared to traditional microbial diagnostic methods for the detection of P. aeruginosa.

We provide a comprehensive genome analysis of Pseudomonas benzopyrenica Ch9–16, a bacterial strain isolated from chili powder processed in Fresnillo, Zacatecas, Mexico. The genome was sequenced using the Illumina NovaSeq platform. Bioinformatic analyses, including assembly and annotation, were conducted on the BV-BRC platform. The genome, comprising 18 contigs and approximately 5.3 million base pairs with a GC content of 65.19 % and 4854 protein-coding sequences. Analysis identified multiple antibiotic resistance genes (ARGs) and virulence genes. This genome data serves as a valuable resource for understanding this bacterial specie and contributes to the database of microorganisms isolated from chili powder. The genome data was deposited at National Center for Biotechnology Information (NCBI) under accession number Bioproject ID PRJNA1062060, Bio Sample ID SAMN45486407. The genome accession number was JBLHDL000000000.

Klebsiella pneumoniae Ch1-39 was isolated from chili powder elaborated at San Luis Potosí, México. This microorganism can be found in diverse ecological niches as water, soil, air, plants and hospital setting, it is considered as a relevant opportunistic pathogen causing several diseases and showing increasingly multi-resistance to antibiotics. The genome was sequenced on the Illumina NovaSeq platform and bioinformatic analyses were made at the Bacterial and Viral Bioinformatics Resource Center (BV-BRC). The genome consisted of 72 contigs with a total size of 5,410,125 bp, 5,361 protein coding sequences (CDS), a total of 6 rRNA and 76 tRNA with an average G + C content of 57.22 %. The genome data was deposited at National Center for Biotechnology Information (NCBI) under accession number Bioproject ID PRJNA1062060, Bio Sample ID SAMN40269967. The genome accession number was JBAWUH000000000.