Viral infections are becoming the foremost driver of morbidity, mortality and economic loss all around the world. Treatment for diseases associated to some deadly viruses are challenging tasks, due to lack of infrastructure, finance and availability of rapid, accurate and easy-to-use detection methods or devices. The emergence of biosensors has proven to be a success in the field of diagnosis to overcome the challenges associated with traditional methods. Furthermore, the incorporation of aptamers as bio-recognition elements in the design of biosensors has paved a way towards rapid, cost-effective, and specific detection devices which are insensitive to changes in the environment. In the last decade, aptamers have emerged to be suitable and efficient biorecognition elements for the detection of different kinds of analytes, such as metal ions, small and macro molecules, and even cells. The signal generation in the detection process depends on different parameters; one such parameter is whether the labelled molecule is incorporated or not for monitoring the sensing process. Based on the labelling, biosensors are classified as label or label-free; both have their significant advantages and disadvantages. Here, we have primarily reviewed the advantages for using aptamers in the transduction system of sensing devices. Furthermore, the labelled and label-free opto-electrochemical aptasensors for the detection of various kinds of viruses have been discussed. Moreover, numerous globally developed aptasensors for the sensing of different types of viruses have been illustrated and explained in tabulated form.

Nanobiosensors are devices that utilize a very small probe and any form of electrical, optical, or magnetic technology to detect and analyze a biochemical or biological process. With an increasing population today, nanobiosensors have become the broadly used electroanalytical tools for the timely detection of many infectious (dengue, hepatitis, tuberculosis, leukemia, etc.) and other fatal diseases, such as prostate cancer, breast cancer, etc., at their early stage. Compared to classical or traditional analytical methods, nanobiosensors have significant benefits, including low detection limit, high selectivity and sensitivity, shorter analysis duration, easier portability, biocompatibility, and ease of miniaturization for on-site monitoring. Very similar to biosensors, nanobiosensors can also be classified in numerous ways, either depending on biological molecules, such as enzymes, antibodies, and aptamer, or by working principles, such as optical and electrochemical. Various nanobiosensors, such as cyclic voltametric, amperometric, impedimetric, etc., have been discussed for the timely monitoring of the infectious and fatal diseases at their early stage. Nanobiosensors performance and efficiency can be enhanced by using a variety of engineered nanostructures, which include nanotubes, nanoparticles, nanopores, self-adhesive monolayers, nanowires, and nanocomposites. Here, this mini review recaps the application of two-dimensional (2D) materials, especially graphitic carbon nitride (g-C₃N₄), graphene oxide, black phosphorous, and MXenes, for the construction of the nanobiosensors and their application for the diagnosis of various infectious diseases at very early stage.

A monolayer of boron known as borophene has emerged as a novel and fascinating two-dimensional (2D) material with exceptional features, such as anisotropic metallic behavior and supple mechanical and optical capabilities. The engineering of smart functionalized opto-electric 2D materials is essential to obtain biosensors or biodevices of desired performance. Borophene is one of the most emerging 2D materials, and owing to its excellent electroactive surface area, high electron transport, anisotropic behavior, controllable optical and electrochemical properties, ability to be deposited on thin films, and potential to create surface functionalities, it has recently become one of the sophisticated platforms. Despite the difficulty of production, borophene may be immobilized utilizing chemistries, be functionalized on a flexible substrate, and be controlled over electro-optical properties to create a highly sensitive biosensor system that could be used for point-of-care diagnostics. Its electrochemical properties can be tailored by using appropriate nanomaterials, redox mediators, conducting polymers, etc., which will be quite useful for the detection of biomolecules at even trace levels with a high sensitivity and less detection time. This will be quite helpful in developing biosensing devices with a very high sensitivity and with less response time. So, this review will be a crucial foundation as we have discussed the basic properties, synthesis, and potential applications of borophene in nanobiosensing, as well as therapeutic applications.