This study provides a comprehensive and refined framework for the micropropagation of the critically endangered Greek endemic Silene conglomeratica. Using a limited seed stock, a reliable in vitro propagation protocol was developed, supporting both ex situ conservation and potential commercial applications in floriculture and landscape architecture. Nodal explants excised from aseptic seedlings, established on half-strength Murashige and Skoog (MS) medium, were successfully used for culture initiation. Supplementation with 1.0 mg L⁻¹ meta-topolin (mT) and 0.2 mg L⁻¹ 1-naphthaleneacetic acid (NAA) promoted shoot proliferation. Subsequent subculturing on half-strength Rugini Olive Medium (OM/2) supplemented with 0.5 mg L⁻¹ 2-isopentenyladenine (2iP) resulted in high multiplication rates and a high frequency of spontaneous rooting. Rooting initiation was further optimized using OM/2 supplemented with 0.5 mg L⁻¹ indole-3-butyric acid (IBA). The high acclimatization percentage (80%) confirms the feasibility of this protocol for ex situ conservation and highlights its applicability for nursery production and specialized landscape use. Overall, this study contributes an efficient and scalable propagation strategy that supports both the conservation and sustainable utilization of this valuable endemic species. Future work should focus on refining these protocols through more targeted testing of concentrations and alternative combinations of growth regulators and nutrient compositions.

Climate change increasingly threatens Mediterranean ornamental horticulture and green infrastructure through elevated temperatures, prolonged drought conditions, soil salinity, and more frequent extreme weather events. As a result, plant growth, phenology and landscape sustainability are significantly affected. This systematic review aimed to identify and qualitatively synthesize the available evidence on the responses of ornamental plants and their production and end use systems to climate-related stress, with emphasis on Mediterranean native species and their potential contribution to climate-resilient landscaping. The review was conducted and reported in accordance with PRISMA 2020. An adapted Population–Exposure–Outcome framework was used to operationalize the overarching review question and guide eligibility assessment. Scopus and the Web of Science Core Collection were systematically searched for peer-reviewed English-language articles published between 1 January 2001 and 31 May 2026. Eligible publications examined ornamental plants, floricultural species, or native and endemic taxa with potential ornamental or landscape use and addressed climate-related stressors, plant resilience, adaptation strategies, cultivation or propagation practices, green-infrastructure applications, or related ecological trade-offs in Mediterranean-relevant contexts. Two reviewers independently assessed titles, abstracts, and full texts using predefined eligibility criteria. The review used a structured qualitative narrative synthesis organized into thematic domains to systematically identify, select, and synthesize the available evidence. Meta-analysis was not undertaken because of substantial heterogeneity in plant material, environmental stressors, study designs, and reported outcomes. A total of ninety studies were included and organized into five domains: climate stress and plant responses (n = 14), native Mediterranean ornamental species (n = 21), adaptation and resilience strategies (n = 16), urban landscaping and green infrastructure (n = 25), and ecological risks and invasive species (n = 14). The review revealed that several native Mediterranean plants possess morphological, physiological, or ecological characteristics associated with tolerance to drought, salinity, and other climate-related stresses, supporting their potential use in sustainable ornamental horticulture. Water-efficient irrigation, alternative water sources and substrates, nursery preconditioning, non-microbial biostimulants, and genotype or physiological screening showed adaptation potential, but their effectiveness depended on species, genotype, intervention intensity, and application context. Evidence remained limited for compound stresses, combined interventions, nursery-to-landscape transfer, long-term field performance, commercial scalability, and environmental trade-offs. Overall, climate-resilient ornamental horticulture requires the integration of plant selection, propagation, production, controlled stress screening, landscape validation, and ecological-risk assessment. This review proposes an evidence-to-application framework to support research, nursery production, landscape planning, and the responsible deployment of climate-adapted ornamental plants.

ISHS III International Symposium on Greener Cities: Improving Ecosystem Services in a Climate-Changing World (GreenCities2024) Participatory design to develop therapeutic gardens in hospital grounds