A holistic approach to daylight dynamics in our built environment can have beneficial outcomes for both physiological and visual effects on humans. Simulations of how daylight variables affect light levels on the horizontal work plane are compared to their physiological effects, measured as melanopic EDI (Melanopic Equivalent Daylight Illuminance) on a vertical plane. The melanopic EDI levels were calculated in a simulated office space in ALFA software (Adaptive Lighting for Alertness) employing the daylight variables of orientation, time of day, season, sky conditions and spatial orientation. Results were analyzed for how daylight design can contribute to the physiological effects of dynamic light in office buildings. Daylight is shown to be a sufficient light source in the majority of cases to meet the recommended values of EDI and provide the suggested horizontal lx level according to the Danish Standards. A mapping of daylight conditions, focusing on the specific factors presented here, can provide guidelines in the design process and future smart building systems. The complex interrelationship between these parameters is important to acknowledge when working with daylight dynamics as a sustainable element in architecture and lighting design.