Global food production faces challenges in balancing the need for increased yields with environmental sustainability. This study presents a six-year field experiment in the North China Plain, demonstrating the benefits of diversifying traditional cereal monoculture (wheat–maize) with cash crops (sweet potato) and legumes (peanut and soybean). The diversified rotations increase equivalent yield by up to 38%, reduce N₂O emissions by 39%, and improve the system’s greenhouse gas balance by 88%. Furthermore, including legumes in crop rotations stimulates soil microbial activities, increases soil organic carbon stocks by 8%, and enhances soil health (indexed with the selected soil physiochemical and biological properties) by 45%. The large-scale adoption of diversified cropping systems in the North China Plain could increase cereal production by 32% when wheat–maize follows alternative crops in rotation and farmer income by 20% while benefiting the environment. This study provides an example of sustainable food production practices, emphasizing the significance of crop diversification for long-term agricultural resilience and soil health.

Grasslands play an important role in conserving natural biodiversity and providing ecosystem functions and services for societies. Soil fertility is an important property in grassland, and the monitoring of soil fertility can provide crucial information to optimize ecosystem productivity and sustainability. Testing various soil physiochemical properties related to fertility usually relies on traditional measures, such as destructive sampling, pre-test treatments, labor-intensive procedures, and costly laboratory measurements, which are often difficult to perform. However, soil enzyme activity reflecting the intensity of soil biochemical reactions is a reliable indicator of soil properties and thus enzyme assays could be an efficient alternative to evaluate soil fertility. Here, we review the latest research on the features and functions of enzymes catalyzing the biochemical processes that convert organic materials to available plant nutrients, increase soil carbon and nutrient cycling, and enhance microbial activities to improve soil fertility. We focus on the complex relationships among soil enzyme activities and functions, microbial biomass, physiochemical properties, and soil/crop management practices. We highlight the biochemistry of enzymes and the rationale for using enzyme activities to indicate soil fertility. Finally, we discuss the limits and disadvantages of the potential new molecular tool and provide suggestions to improve the reliability and feasibility of the proposed alternative.

The Earth’s terrestrial carbon stocks have depleted an estimated 344 billion tons. Carbon losses amid water scarcity in climate-vulnerable drylands are a mounting challenge, and their restoration requires optimizing water-to-carbon biotransformation. Synthesizing thousands of worldwide experimental studies, we identify key biophysical pathways for enhancing carbon restoration and ecosystem resilience in global drylands, as follows: (i) Cropping diversification increases net primary productivity by 18.9% (n = 1296 studies); (ii) Regulated deficit irrigation cuts water use by 30–50% while improving yield-scaled water use efficiency by 3.4% (n = 9068 paired comparisons); (iii) Soil mulching increases land productivity by 22.2% (n = 48,144 paired comparisons); and (iv) Soil health rejuvenation strategies can sequester 1.2–3.8 t SOC ha⁻¹ yr⁻¹. Priorities to implement these biophysical pathways to enhance water-to-carbon biotransformation include: ‘smart’ irrigation, carbon dioxide fertilization-enhanced photosynthetic assimilation, rhizosphere engineering for microbiome-based nutrient solutions, biodegradable mulches replacing traditional polyethylene films, diversifying farming systems with low soil disturbance and climate-smart practices, and inclusive governance frameworks. These prioritized strategies reconcile water scarcity with carbon restoration to enhance dryland ecosystem resilience, which supports the UN’s Sustainable Development Goals.

Meeting global food demands by 2050 requires a 45–60% increase in agricultural production. Plasticulture has emerged as a pivotal yet controversial solution. Here we perform a meta-analysis synthesizing the findings of global studies and reveal that plastic mulch enhances crop yields by 28.7% and water use efficiency by 48.9% under diversified systems. In China (2015–2024), plasticulture contributed an additional 189 million tons (Mt) of staple food, conserved 33.5 million hectares of arable land, and reduced emissions by 438 Mt CO₂-equivalent. However, persistent plastic residues degrade soils, and nanoplastics infiltrate food chains, posing ecological and health risks. Despite global negotiations (2024–2025), a binding UN treaty on plastic pollution remains stalled due to disparities among players. To reconcile productivity with sustainability, we propose six evidence-based priorities: (1) scaling integrated eco-farming systems with AI-driven precise application of soil mulches; (2) accelerating material innovation, focusing on biodegradable films and organic-based alternatives; (3) deploying blockchain-enabled circular economies for plastic waste; (4) improving reuse and recycling infrastructure; (5) implementing localized incentive mechanisms to support plastic-free farming; and (6) integrating plastic management into UN carbon trading frameworks. These strategies can pivot plasticulture toward a climate-resilient, ecologically sustainable model—balancing food security with environmental stewardship in an era of climate uncertainty.

Arbuscular mycorrhizal (AM) fungal symbiosis offers a transformative solution in mitigating agroecosystem challenges linked with synthetic chemical overuse. However, the potential of AM-plant communications in response to anthropogenic activities and hyphal network functionality remains poorly understood. Here, we reposition AM fungal hyphosphere networks as keystone ecological infrastructure for sustainable agroecosystems. Synthesizing thousands of worldwide experimental studies reveals the main environmental functions of AM fungi-plant communication: enhancing agroecosystem resilience by buffering crops against various (a)biotic stressors through molecular signaling and physiological changes; mediating energy transferring via small RNA-mediated cross-kingdom interactions; facilitating hydraulic redistribution in the soil profile via hyphospheric network; and optimizing root architecture via effective colonization for nutrient acquisition. Some anthropogenic practices-soil disturbance, non-mycorrhizal crop monoculture, and fungicide use-disrupt AM hyphal networks; however, those can be minimized through improved farming practices, such as cropping diversification with legumes and AM fungi-compatible crops, AM-responsive plant genotypes, effective AM fungal inoculation, and microbial consortia amendments. Bridging AM fungal mechanisms with anthropogenic practices and policy supports is essential to scale AM benefits to various ecoregions. Exploring AM fungal functionality can increase nutrient use efficiency, reduce chemical inputs, and enhance ecosystem productivity, offering a microbial-centric blueprint in helping the UN's sustainability goals.

Global agrifood systems face three interconnected challenges: ensuring food security, promoting environmental sustainability, and restoring soil health in the face of climate change. Conventional practices have prioritized productivity over ecological resilience, leading to soil degradation, increased greenhouse gas (GHG) emissions, and inefficient resource utilization. Here, we introduce a “triple-goal” agrifood framework that enhances food production, soil health, and GHG mitigation simultaneously through integrated innovations. Using a second-order meta-analysis of 104 meta-analyses that cover 39,162 studies and 300,139 global field comparisons, we identified key interventions, including optimized fertigation, diversified cropping systems, organic amendments, and precision N management, that increased productivity by 14%–28% while reducing environmental impacts. Diversified systems boosted yields by 19.6% and reduced land use by 19%. Integrating legumes and cover crops lowered N₂O emissions by 18%–65%, while organic amendments increased soil organic carbon stocks by 7%–13%. Structural equation modeling identified nitrogen use efficiency and microbial activity as central to the food-soil-emissions nexus. However, tradeoffs remain; yield-focused strategies can elevate emissions if not tailored to local conditions. By integrating agronomic, biological, and technological interventions such as conservation tillage, biofertilization, and digital agriculture, this triple-goal framework supports a 15%–30% reduction in anthropogenic CO₂-equivalent emissions. These findings underscore the need for policy reform and multi-stakeholder collaboration to scale up the adaptation of integrated strategies in alignment with the UN’s Sustainable Development Goals and the “One Health” initiative. The triple-goal framework provides a transformative pathway to climate-smart, equitable, and resilient agrifood systems that strike a balance between productivity and planetary health.