In the face of mounting challenges related to limited availability of urban land and ecological degradation, emerging novel ecosystems offer unique opportunities for ecological regeneration, social redefinition of space, and alternative urban visions. This study presents the multi-layer analysis of the Goccia Forest in Milan (Italy), a wild urban woodland that has developed over sealed and polluted post-industrial land, aiming to investigate the potential of this novel ecosystem to sustain Nature-based Solutions (NbSs). Using an integrated approach (surveys on fauna, vascular flora, lichens, analysis of forest evolution, mapping of sealed surfaces, and soil characterization) the research looks at the novel ecosystem as a whole, highlighting its ecological dynamics and Ecosystem Services (ES). La Goccia Forest serves as a prime example of how the implementation of NbSs is intricately intertwined with the spontaneous regeneration of urban brownfields. The present study offers the opportunity to rethink urban policies, ensuring their alignment with the demands of the population and the latest scientific knowledge.