Diptera identification is fundamental in forensic entomology as well as in funerary archeoentomology, where the challenge is exacerbated by the presence of immature stages such as larvae and puparia. In these two developmental stages, specimens possess a very limited number of diagnostic features, and for puparia, there is also a lack of identification tools such as descriptions and identification keys. Morphological analysis, DNA-based techniques, and cuticular chemical analyses all show good potential for species identification; however, they also have some limitations. DNA-based identification is primarily hindered by the incompleteness of genetic databases and the presence of PCR inhibitors often co-extracted from the puparial cuticle. Chemical analysis of the cuticle is showing promising results, but this approach is also limited by the insufficient profile database and requires specific, expensive equipment, as well as trained personnel. Additionally, to ensure the repeatability of the analysis—a critical aspect in forensic investigations—and to preserve precious and unique specimens from museum collections, non-invasive protocols and techniques must be prioritized for species identification.

Insects found in archeological contexts provide useful information for reconstructing past events. In the context of funerary archeology, insects may help in reconstructing funerary practices or rituals, and in the understanding of the taphonomic processes. Furthermore, studying these insects is fundamental for developing effective conservation strategies for cultural heritage. This article focuses on the entomological investigation of four mummies (nineteenth century) discovered in the church of Santa Maria Annunziata (Cerreto di Spoleto, Central Italy). The research aimed to verify and eventually support archeological hypotheses about the four bodies and to plan an effective conservation strategy. The predominant findings were Diptera puparia and adult Coleoptera. Alongside, common species typical of the hypogean burial, such asHydrotaea capensisand several mycetophilous (mold feeder) species were also collected. The presence of blowfly remains (Calliphoridae) would suggest that the bodies were exposed before burial.

Paleoradiology has become a standard diagnostic method in the study of mummified or embalmed bodies. Among the various available techniques, computed tomography valuing for its ability to provide detailed information. However, computed tomography equipment is not always accessible to research teams, cannot be easily transported to all conservation sites, and raises health concerns. Therefore, mobile digital radiographic technology is often the most suitable investigative tool in specific contexts. In this study, three mummies preserved at the Sanctuary of Madonna della Corona, perched on a cliff above the Adige River Valley on the Monte Baldo at an elevation of 775 m a.s.l., were analyzed using radiographic techniques. The impossibility of transferring the bodies due to the sanctuary’s remote location imposed the use of lightweight, portable equipment for the radiological examination. This article highlights next-generation X-ray technology utility, potential, and limitations in investigating clothing fabrics, restoration history, taphonomy, entomology, anthropology, and paleopathology. The interdisciplinary approach in this study has unveiled new historical and biological insights about these mummies, which, though revered in popular devotion, had previously been unknown to both Italian and international scientific communities.

Funerary archaeoentomology is the discipline that studies insects and other arthropods in archaeological contexts, with a particular focus on the funerary domain. The presence of specific species, such as necrophagous beetles or saprophagous flies, can provide crucial evidence regarding post-mortem conditions—whether bodies were left exposed to the air or buried suddenly after death—and whether they underwent particular preservation practices, such as desiccation or embalming. This study concentrates on entomological specimens collected from three mummified bodies at the Sanctuary of Madonna della Corona in the province of Verona (northeast Italy), aiming to reconstruct aspects of funerary practices, especially the season of death and the authenticity of the garments worn by the mummified individuals. Insects were manually collected from bodies belonging to three hermits living between the 17th and 19th centuries. A complex entomofauna consisting of Diptera, Coleoptera, Lepidoptera, and minor taxa was collected and analyzed. Diptera puparia, primarily from the families Calliphoridae, Muscidae, and Fanniidae, were the most abundant entomological elements recovered. Their presence suggests potential exposure of the bodies before burial and indicates that death likely occurred during a mild period of the year (end of spring/beginning of autumn). The co-occurrence of holes caused by maggots on the hermits’ skin and their garments allows us to speculate about the authenticity of the clothing used during the funerary rituals. By combining entomological evidence with textile analysis, this research offers a more precise understanding of historical funerary practices within this devotional context. It sheds light on methods of managing human remains, burial traditions, and preservation techniques, particularly regarding the clothing of the deceased.