Takashi Hirase

Dr. Takashi Hirase

Orthopaedic Surgery, UCSD School of Medicine, La Jolla, USA
orcid

Research Focus & Metrics

Academic Career Length

14Years (2013-Present)

(Calculated once Scilit publication data is synced: current year − year of first publication + 1)

Representative Publications

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4 February 2013 in Genome Research
Verified
Journal Article
Open access
PDF file
GVGaurav K. Varshney
Gaurav K. Varshney
JLJing Lu
Jing Lu
DGDerek E. Gildea
Derek E. Gildea
HHHaigen Huang
Haigen Huang
WPWuhong Pei
Wuhong Pei
SLShuo Lin
Shuo Lin
With the completion of the zebrafish genome sequencing project, it becomes possible to analyze the function of zebrafish genes in a systematic way. The first step in such an analysis is to inactivate each protein-coding gene by targeted or random mutation. Here we describe a streamlined pipeline using proviral insertions coupled with high-throughput sequencing and mapping technologies to widely mutagenize genes in the zebrafish genome. We also report the first 6144 mutagenized and archived F1's predicted to carry up to 3776 mutations in annotated genes. Using in vitro fertilization, we have rescued and characterized ∼0.5% of the predicted mutations, showing mutation efficacy and a variety of phenotypes relevant to both developmental processes and human genetic diseases. Mutagenized fish lines are being made freely available to the public through the Zebrafish International Resource Center. These fish lines establish an important milestone for zebrafish genetics research and should greatly facilitate systematic functional studies of the vertebrate genome.

1 June 2021 in The Spine Journal
Verified
Journal Article
Closed access
Takashi HiraseTakashi Hirase
Takashi Hirase
VHVaran Haghshenas
Varan Haghshenas
RBRachel Bratescu
Rachel Bratescu
DDDavid Dong
David Dong
PKPeggy H. Kuo
Peggy H. Kuo
RMRex A.W. Marco
Rex A.W. Marco

15 October 2018 in World Journal of Diabetes
Verified
Journal Article
Open access
Takashi HiraseTakashi Hirase
Takashi Hirase
EREric Ruff
Eric Ruff
Salim SuraniSalim Surani
Salim Surani
IRIqbal Ratnani
Iqbal Ratnani
Topical application of platelet-rich plasma for diabetic foot ulcers: A systematic review

9 April 2019 in Arthroscopy
Verified
Journal Article
Closed access
RJRobert A. Jack
Robert A. Jack
KSKyle R. Sochacki
Kyle R. Sochacki
Takashi HiraseTakashi Hirase
Takashi Hirase
JVJustin W. Vickery
Justin W. Vickery
JHJoshua D. Harris
Joshua D. Harris

16 October 2018 in Current Reviews in Musculoskeletal Medicine
Verified
Journal Article
Closed access
PDF file
SESamuel O. Ewalefo
Samuel O. Ewalefo
MDMalcolm Dombrowski
Malcolm Dombrowski
Takashi HiraseTakashi Hirase
Takashi Hirase
JRJorge L. Rocha
Jorge L. Rocha
MWMitchell Weaver
Mitchell Weaver
MHMaCalus V. Hogan
MaCalus V. Hogan

31 October 2017 in Arthroscopy
Verified
Journal Article
Closed access
KSKyle R. Sochacki
Kyle R. Sochacki
RJRobert A. Jack
Robert A. Jack
Takashi HiraseTakashi Hirase
Takashi Hirase
PMPatrick C McCulloch
Patrick C McCulloch
DLDavid M. Lintner
David M. Lintner
JHJoshua D. Harris
Joshua D. Harris