Acute thrombosis remains the main limitation of small-diameter vascular grafts (inner diameter <6 mm) for bridging and bypassing of small arteries defects and occlusion. The use of hydrogel tubes represents a promising strategy. However, their low mechanical strength and high swelling tendency may limit their further application. In the present study, a hydrogel vascular graft of Ca alginate/polyacrylamide reinforced with a braided fiber strut was designed and fabricated with the assistance of a customized casting mold. Morphology, structure, swellability, mechanical properties, cyto- and hemocompatibility of the reinforced graft were characterized. The results showed that the reinforced graft was transparent and robust, with a smooth surface. Scanning electron microscopic examination confirmed a uniform porous structure throughout the hydrogel. The swelling of the reinforced grafts could be controlled to 100%, obtaining clinically satisfactory mechanical properties. In particular, the dynamic circumferential compliance reached (1.7 ± 0.1)%/100 mmHg for 50–90 mmHg, a value significantly higher than that of expanded polytetrafluoroethylene (ePTFE) vascular grafts. Biological tests revealed that the reinforced graft was non-cytotoxic and had a low hemolysis percentage (HP) corresponding to (0.9 ± 0.2)%. In summary, the braided fiber-reinforced hydrogel vascular grafts demonstrated both physical and biological superiority, suggesting their suitability for vascular grafts.

As a promising agent for biomedical application, collagen has been used as a nanofiber to architecturally mimic its fibrillar structure in Extracellular Matrix (ECM); however, it has to be modified by techniques, such as crosslinking, to overcome its limitations in structural stability along with potential toxicity. Here, we prepared collagen/polyethylene oxide (PEO) nanofibrous membranes with varying crosslinking degrees and their properties, such as water stability, mechanical properties, blood clotting capacity and cytocompatibility, were studied systematically. By investigating the relationship between crosslinking degree and their properties, nanofibrous membranes with improved morphology retention, blood clotting capacity and cytocompatibility have been achieved. The result of circular dichroism measurement demonstrated that a triple helical fraction around 60.5% was retained. Moreover, the electrospun collagen/PEO at crosslinking degrees above 60.6% could maintain more than 72% of its original weight and its nanofibrous morphology under physiological conditions could be well preserved for up to 7 days. Furthermore, the crosslinked collagen/PEO membrane could provide a more friendly and suitable environment to promote cell proliferation, and about 70% of the clot can be formed in 5 min. With its superior performance in water stability, hemostasis and cytocompatibility, we anticipate that this nanofibrous membrane has great potential for wound dressing.

Background: Cosmc (C1GalT1C1) mutation could cause aberrant O-glycosylation and result in expression of Tn antigen on the surface of tumor cells (Tn⁺ cells), which is associated with the metastasis and prognosis of cancer progression. Mesenchymal stem cells (MSCs) could participate in immunoregulation, tissue damage repair, and tumor inhibition and be seen as an ideal candidate for tumor therapy due to their inherent capacity to migrate to tumor sites. However, their therapeutic effectiveness in different tumors is inconsistent and still controversial. Of note, emerging data reveal that side population (SP) cells have a stronger multilineage developmental potential than main population cells and can function as stem/progenitor cells. The effect of SP cells derived from MSCs on the biological behaviors and the O-glycosylation status of tumor cells remains unclear. Methods: SP cells were isolated from human umbilical cord MSCs (hUCMSCs) and human placenta MSCs (hPMSCs). Tn⁺ cells (LS174T-Tn⁺ and HT-29-Tn⁺ cells) and matching Tn⁻ cells (LS174T-Tn⁻ and HT-29-Tn⁻ cells) were isolated from human colorectal cancer cell (CRC) lines LS174T and HT-29 by immune magnetic beads. The proliferation, migration, apoptosis, Tn antigen expression, and O-glycome in Tn⁺ and Tn⁻ CRC cells before and after co-cultured with SP-MSCs were detected using real-time cell Analysis (RTCA), flow cytometry (FCM), and cellular O-glycome reporter/amplification (CORA), respectively. Cosmc protein and O-glycosyltransferase (T-synthase and C3GnT) activity in CRC cells were, respectively, assessed using western blotting and fluorescence method. Results: Both SP cells derived from hUCMSCs and hPMSCs could inhibit proliferation and migration, promote apoptosis of CRC cells, significantly reduce Tn antigen expression on Tn⁺ CRC cells, generate new core 1-, 2-, and 3-derived O-glycans, increase T-synthase and C3GnT activity, and elevate the levels of Cosmc and T-synthase protein. Conclusion: SP-hUCMSCs and SP-hPMSCs could inhibit proliferation and migration and promote apoptosis of Tn⁺ CRC cells via increasing O-glycosyltransferase activity to modify O-glycosylation status, which further adds a new dimension to the treatment of CRC.

Cadherins are surface glycoproteins on plasma membranes and exist in many forms: T-cadherin, neuronal cadherin (N-cadherin), epithelial cadherin (E-cadherin), and vascular endothelial (VE-cadherin). Cadherins play critical roles in cell–cell interactions and are involved in multiple functions related to cell growth and proliferation. Findings from numerous reports have indicated that VE-cadherin regulates the remodeling, gating, and maturation of vascular vessels. The surface morphology of materials also impacts endothelial cell adhesion. This report is an overview of recent research on the effects of cadherins on cell–cell interactions, along with cell adhesions as examined on different materials. This summary will provide novel insights and approaches for research on cell–cell and cell–material interactions and illuminate some of the mechanisms of cell growth on different materials.