Research on human nail tissue has been limited by the restricted access to fresh specimen. Here, we studied transcriptome profiles of human nail units using polydactyly specimens. Single-cell RNAseq with 11,541 cells from 4 extra digits revealed nail-specific mesenchymal and epithelial cell populations, characterized by RSPO4 (major gene in congenital anonychia) and SPINK6, respectively. In situ RNA hybridization demonstrated the localization of RSPO4, MSX1 and WIF1 in onychofibroblasts suggesting the activation of WNT signaling. BMP-5 was also expressed in onychofibroblasts implicating the contribution of BMP signaling. SPINK6 expression distinguished the nail-specific keratinocytes from epidermal keratinocytes. RSPO4⁺ onychofibroblasts were distributed at close proximity with LGR6⁺ nail matrix, leading to WNT/β-catenin activation. In addition, we demonstrated RSPO4 was overexpressed in the fibroblasts of onychomatricoma and LGR6 was highly expressed at the basal layer of the overlying epithelial component, suggesting that onychofibroblasts may play an important role in the pathogenesis of onychomatricoma.

Hwang HW, et al. Ann Dermatol. 2024 Feb;36(1):44-52. https://doi.org/10.5021/ad.23.082

Park H, et al. Ann Dermatol. 2023 Jun;35(3):205-216. https://doi.org/10.5021/ad.22.167

Lee SB, et al. Ann Dermatol. 2024 May;36:e40. https://doi.org/10.5021/ad.23.128