Background/Objectives: Oreganum vulgare essential oil (OEO) is safe, effective, multifunctional, and widely used. This study aimed to evaluate OEO’s chemical composition and antimicrobial activity in vitro against S. aureus and E. coli. Methods: The composition of OEO was determined by gas chromatography–mass spectrometry (GC-MS). Results: Compounds included monoterpenes with known antimicrobial activity, such as 2-menthen-1-ol (36.33%), linalyl acetate (9.26%), terpinene-4-ol (9.01%), 4-thujanol (6.33%), menthen (5.81%), sabinene (5.18%), and carvacrol methyl ether (5.14%). Conclusions: OEO had a strong antimicrobial activity with a minimum inhibitory concentration (MIC) of 1.90 mg/mL for S. aureus and 0.49 mg/mL for E. coli after 18 h incubation. The minimum bactericidal concentration (MBC) was 7.9 mg/mL against S. aureus and 0.99 mg/mL against E. coli. Thus, OEO could be used as a natural antimicrobial against S. aureus and E. coli infections.

Enzymatic electrochemical biosensors play an important role in the agri-food sector due to the need to develop sustainable, low-cost, and easy-to-use analytical devices. Such biosensors can be used to monitor pathogens, endocrine disruptors, and pesticides, such as carbaryl, widely used in many crops. The use of renewable carbon (RC) sources, provided from biomass pyrolysis has been often applied in the fabrication of such sensors. This material is a great candidate for biosensor fabrication due to the presence of surface functional groups, porosity, and moderate surface area. This work describes the functionalization of RC material through an acid treatment with a sulfonitric solution HNO₃/H₂SO₄ (1:3) and the resulting material was characterized by scanning electron microscopy. The obtained RC functionalized (RCF) and the acetylcholinesterase enzyme (AChE) were applied in the construction of the electrochemical biosensor on glassy carbon (GC) electrode and used to detect carbaryl in apple samples. The GC/RCF/AChE biosensor was able to detect the carbaryl pesticide from 5.0 to 30.0 nmol L⁻¹, displaying a LOD of 4.5 nmol L⁻¹. The detection of carbaryl in apple samples presented recoveries between 102.5 to 118.6% through the standard addition method. The proposed biosensor is a promising renewable tool for food safety.

Climate change and population growth have heavily impacted the ecosystem’s water resources, essential for anthropogenic activities. These also apply to the Andean city of Chachapoyas, located in the north of Peru, which has gone through a substantial population increase in recent years, therefore increasing its water demand. This research aimed to assess dry and wet events from 1981 to 2019 that have taken place in Chachapoyas, by applying the Standardized Precipitation Index (SPI), and the Standardized Precipitation Evapotranspiration Index (SPEI). These events were periodically characterized, and the index relationship was determined at different timescales. The SPI and SPEI indices were calculated at the city’s only weather station for timescales of 3, 6, 12, and 24 months using climatic data. The indices showed a remarkably consistent behavior for timescales of 12 and 24 months detecting an extreme drought event in 1993, while for timescales of 3 and 6 months a severe drought event was detected in the same year. Contrastingly, there has been an increase in extreme wet events in the last decade, hence Chachapoyas is categorized between "moderate drought" and “moderate wet”. It should be noted that the indices have a high correlation between them when calculated for the same timescale. The results were statistically significant (p < 0.05). Considering the results obtained related to dry and wet events and their relation with economic activities such as environmental management, we can conclude that the SPI and SPEI indices are useful and valuable tools for local and regional governments.

Traditional knowledge (TK) of medicinal plants in cities has been poorly studied across different inhabitants’ socioeconomic sectors. We studied the small city of Chachapoyas (~34,000 inhabitants) in the northern Peruvian Andes. We divided the city into three areas according to the socio-economic characteristics of its inhabitants: city center (high), intermediate area (medium), and city periphery (low). We gathered information with 450 participants through semi-structured interviews. Participants of the city periphery showed a higher TK of medicinal plants than participants of the intermediate area, and the latter showed a higher TK than participants of the city center. The acquisition of medicinal plants was mainly through their purchase in markets across the three areas, although it was particularly relevant in the city center (94%). Participants of all socioeconomic levels widely used the same medicinal plants for similar purposes in Chachapoyas, which is likely based on a common Andean culture that unites their TK. However, participants with the lowest socioeconomic level knew and used more plants for different medicinal uses, indicating the necessity of these plants for their livelihoods. City markets with specialized stores that commercialize medicinal plants are key to preserve the good health of poor and rich people living in Andean cities and societies.

FUENTES DE CONTAMINACIÓN ESTACIONALES EN LA CUENCA DEL RÍO UTCUBAMBA, REGIÓN AMAZONAS, PERÚ

Anthropic activity affects the hydrogeomorphological quality of fluvial systems. River and valley classifications are fundamental preliminary steps in determining their ecological status, and their prioritization is essential for the proper planning and management of soil and water resources. Given the importance of the High Andean livestock micro-watershed (HAL-MWs) ecosystems in Peru, an integrated methodological framework is presented for morphometric prioritization that uses a Principal Component Analysis (PCA) and Weighted Sum Approach (WSA), geomorphological fluvial classifications (channel, slope, and valley), and hydrogeomorphological evaluations using the Hydrogeomorphological Index (IHG). Of six HAL-MWs studied in Leimebamba and Molinopampa (Amazonas region), the PCWSA hybrid model identified the San Antonio HAL-MW as a top priority, needing the rapid adoption of appropriate conservation practices. Thirty-nine types of river course were identified, by combining 13 types of valley and 11 types of riverbed. The total assessment of the IHG indicated that 7.6% (21.8 km), 14.5% (41.6 km), 27.9% (80.0 km), and 50.0% (143.2 km) of the basin lengths have “Poor”, “Moderate”, “Good”, and “Very good” quality rankings, respectively. The increase in the artificial use of river channels and flood plains is closely linked to the decrease in hydrogeomorphological quality.