Kiwifruit (Actinidia spp.) postharvest decay is common in China, which can cause serious economic losses to kiwifruit industry. In order to further clarify the pathogen of kiwifruit rot disease in Guizhou Province, the rotten fruits of kiwifruit (cultivar Jinyan) were collected, and the pathogenic fungi were identified by isolation and purification, pathogenicity test, morphological characteristics, and analysis of rDNA-ITS sequences. The results showed that the pathogenic fungi of kiwifruit rot disease were Diaporthe phaseolorum and Fusarium tricinctum. Meanwhile, the results showed that all the tested agents had a certain inhibitory effect on Diaporthe phaseolorum and Fusarium tricinctum. Among them, 33.5 quinolone SC had the best inhibitory effect on Diaporthe phaseolorum with an EC₅₀ value of 9.67mg/L, and 25 fludioxonil SC had the best inhibitory effect on Fusarium tridentatus with the EC₅₀ value of 13.13mg/L. The results will provide a reference for the control of kiwifruit rot disease.

Kiwifruit is an important economic crop in the world today with a high nutritional value. It can cause huge damage by causing kiwifruit rot disease; however, at present, the control methods for this disease are limited. In this study, the rotten fruits of kiwifruit (Cultivar Jinyan) were collected from Pujiang city (Sichuan province), Xixia city, (Henan province), Zhouzhi (Shaanxi province), Meixian city (Shaanxi province), and Bijie (Guizhou province), China, and the pathogenic fungi were identified by isolation and purification, pathogenicity test, morphological characteristics, and analysis of ribosomal DNA internal transcribed spacer (rDNA-ITS) sequences. The results showed that the pathogenic fungi of kiwifruit rot disease were Botryosphaeria dothidea and Dothiorella gregaria. Meanwhile, the in vitro antifungal activity of 11 kinds of fungicides and 5 kinds of plant essential oils against B. dothidea and D. gregaria were determined and the results showed that all the tested fungicides and plant essential oils had a certain inhibitory effect on B. dothidea and D. gregaria. Among them, propiconazole had the best inhibitory effect on B. dothidea with an EC₅₀ value of 4.10mg/L, and quinolinone had the best inhibitory effect on D. gregaria with the EC₅₀ value of 10.05mg/L. Moreover, the pesticides and essential oils have practical application values for prevention and treatment of fruit rot diseases pathogens.

Except for a few stick insects that are economically valuable, most species be considered to be forest pests, so it is extremely important to obtain plant host-use information of more stick insects. In this paper, the plant hosts of three species of stick insects were recorded for the first time. We also discovered these stick insects can feed upon the flowers or leaves of plants. Lopaphus unidentatus (Chen & He, 1995) (Phasmida: Lonchodidae) attacked Hypericum choisianum Wall. ex N. Robson, 1973 (Hypericaceae), Leurophasma dolichocercum Bi, 1995 (Phasmida: Aschiphasmatidae) attacked Antenoron filiforme (Thunb.) Roberty & Vautier, 1964 (Polygonaceae) and Megalophasma granulatum Bi, 1995 (Phasmida: Lonchodidae) attacked Debregeasia orientalis C. J. Chen, 1991 (Urticaceae). Finally, we were lucky enough to also obtain photographs of them mating and feeding.

Accurate taxonomical identification is an extremely important basis for stick insect research, including evolutionary biology but also applied biology such as pest control. In addition, genetic methods are a valuable identification auxiliary technology at present. Therefore, this paper used morphological and molecular data to investigate five stick insect specimens from the genus Cnipsomorpha in Yunnan, successfully identifying two new species: Cnipsomorpha yunnanensis Xu, Jiang & Yang, sp. nov. and C. yuxiensis Xu, Jiang & Yang, sp. nov. A phylogenetic tree was constructed through their 28S and COI genes in order to infer the phylogenetic position of the two new species. Photographs of the new species and a key to all known Cnipsomorpha species are provided.