Voltage-gated potassium (Kv) channels are known to be involved in cancer development and cancer cell proliferation. KV9.3, an electronically silent subunit, forms heterotetramers with KV2.1 in excitable cells and modulates its electrophysiological properties. However, the role of KV9.3 alone in non-excitable cancer cells has not been studied. Here, we evaluated the effect of silencing KV9.3 on cancer cell proliferation in HCT15 colon carcinoma cells and A549 lung adenocarcinoma cells. We confirmed the expression of KV9.3 mRNA in HCT15 and A549 cells and showed that silencing KV9.3 using small interfering RNA caused G0/G1 cell cycle arrest and alterations in cell cycle regulatory proteins in both HCT15 and A549 cells without affecting apoptosis. Also, stable knockdown of KV9.3 expression using short-hairpin RNA inhibited tumor growth in SCID mouse xenograft model. Using a bioinformatics approach, we identified Sp1 binding sites in the promoter region of the gene encoding KV9.3. We further found that Sp1 bound to this region and showed that the Sp1 inhibitor, mithramycin A, induced a concentration-dependent decrease in KV9.3 expression. Taken together, these data suggest that knockdown of KV9.3 inhibits proliferation in colon carcinoma and lung adenocarcinoma cell lines and may be regulated by Sp1.

The industry foundation classes (IFC) data model is the most important data schema in ensuring the interoperability of the information generated throughout the lifecycle of facilities. However, because the current IFC model is focused on buildings, there are limitations when this model is applied to bridge structures. This paper proposes a method that enables the information modeling of steel box girder bridges based on the current IFC. To select the required and core items, we classify the components of a steel box girder bridge by the design stage with reference to engineering documents. To generate functional meanings of each bridge component, we develop the rules of the unique identifier and information reassignment, and then apply a semi-automated naming algorithm. The generated bridge information model was used to confirm the functional semantic meanings of individual components, and it was checked whether additional external information, such as carbon emissions, could be linked for specific bridge components. It was observed that information retrieval and extraction for components is possible through a semantic-based query to the generated IFC-based bridge information model.

A tunnel data schema employing the New Austrian Tunneling Method (NATM) is proposed to efficiently manage and exchange the design information of road tunnels between different computer environments. The data schema was developed by extending the Industry Foundation Classes (IFC) used as standards in Building Information Modeling/Model (BIM) projects. The design guidelines and tunnel drawings were analyzed in order to apply the design information of the NATM road tunnels. From the analysis results, the applicable IFC elements were identified through a comparative analysis with the existing IFC data schema, and new items were defined for the additional necessary elements. The newly defined elements were classified into the parts representing the information of the space occupied by the tunnel and that representing the information on components such as shotcrete, steel ribs, and concrete linings. The applicability of the developed schema was examined by using it to construct an experimental environment, in which information modeling was possible.

This paper presents an experiment-based synthetic structural analysis method that combines digital image processing (DIP) and the particle difference method (PDM), which is a strong form-based meshfree method. The proposed method uses images to determine the displacement of deformed specimens, interpolates the displacement onto nodes of the PDM model without meshes or grids, and calculates the kinematic variables. Furthermore, the pixel extraction method for the target area and the method of setting the region of interest for expediting DIP were used during the synthetic structural analysis. A method for effectively expanding the number of tracking points and an improved method for labeling tracking points are also presented. To verify the performance of the analysis, the experimental and numerical analysis results of a three-point bending test on a rubber beam were compared in terms of various mechanical variables as well as with the PDM results of a simulated bending test. It was found that tracking point expansion and adjusting the radius of the domain of influence are advantageous for performing an accurate calculation without losing computational efficiency. It was demonstrated that the synthetic structural analysis effectively overcomes the shortcomings of the conventional experiments and the limitations of pure simulations.

75 1. 서 론 콘크리트 구조물에서 발생하는 균열은 구조물의 안전성과

한국전산구조공학회 논문집 제37권 제1호(2024.2) 67 1. 서 론 구조물의 생애주기 상에서 효과적인 유지관리나 새로운 구 조형식을 개발하는 과정에서 구조적 부재의 변형, 응력, 변형 률 같은 운동 역학적 변수들을 직접 측정하거나 해석적인 방법 으로 정확하게 추정하는