Motor and physical fitness play a crucial role to optimize the player performance in the soccer game. Implementing a customized workout schedule targeting Motophysic Fitness (MPF) helps to elevate their on-field performance. The present study was aimed to assess the impact of a 12-week MPF Training program targeted to have improvements in physical fitness for elite soccer players. MPF training program was implemented during the off-season for youth soccer players with average age (20 ± 1 years), height (1.75 ± 0.5 m) weight (64.3 ± 5.7 kg). Soccer-related fitness traits were assessed to evaluate performance levels, while Electroencephalography (EEG) was utilized for cognitive assessment. Statistical analysis was performed to evaluate the outcome of the fitness traits. Significant improvements were observed across diverse fitness traits, with mean values increasing by a minimum of 2 % and a maximum of 5 %. Speed emerged as a predominant contributor, showing a robust correlation (Adjusted R² = 0.84), while agility, strength, power, endurance, balance, coordination, and reaction time also displayed substantial improvements. Despite observable gains in flexibility, its impact on overall fitness appeared comparatively modest (Adjusted R² = 0.23). Z-test confirmed the statistical significance of all fitness assessments post-MPF, with p-values less than 0.05 for each test. Features extracted from EEG highlighted the improvement in cognitive ability before and after training.