Lucia Lanza

Dr. Lucia Lanza

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Research Focus & Metrics

Academic Career Length

8Years (2019-Present)

(Calculated once Scilit publication data is synced: current year − year of first publication + 1)

Representative Publications

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12 November 2019 in Applied Microbiology and Biotechnology
Verified
Journal Article
Closed access
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SNSilvia Adriana Navarro
Silvia Adriana Navarro
Lucia LanzaLucia Lanza
Lucia Lanza
Natalia Rios ColomboNatalia Rios Colombo
Natalia Rios Colombo
MUM. Fernandez de Ullivarri
M. Fernandez de Ullivarri
LALeonardo Acuña
Leonardo Acuña
MCMiriam C. Chalón
Miriam C. Chalón


Verified
Journal Article
Open access
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EMEmilse Masias
Emilse Masias
JRJuan Ignacio Ramirez
Juan Ignacio Ramirez
Lucia LanzaLucia Lanza
Lucia Lanza
JLJorge A. Lachenicht
Jorge A. Lachenicht
MVMaria Elisa Vazquez
Maria Elisa Vazquez
RSRaul Armando Salomón
Raul Armando Salomón
A comprehensive analysis of the microcin J25 (MccJ25) biosynthetic gene cluster revealed a previously uncharacterized 96-base pair overlapping gene, designated mcjX. This gene features a +1 reading frame shift relative to the primary sequence and encodes a 31-amino acid peptide. Notably, 53 nucleotides overlap with the 3′ terminus of the structural gene mcjA. Such significant overlaps are rare features in the Escherichia coli genome, highlighting the hidden complexity of microbial operon architectures. In this study, we demonstrate that mcjX is actively translated. Functional assays, including green fluorescent protein reporter systems, suggest that McjX acts as a negative regulator of the mcjA promoter, modulating MccJ25 expression. This discovery represents the first report of a regulatory mechanism mediated by an overlapping gene within a lasso peptide operon, providing new perspectives on how microbial genomes fine-tune the production of antimicrobial peptides through compact genetic organization.